Antioxidant activity of the melanin pigment extracted from Aspergillus nidulans
Antioxidant activity of the melanin pigment extracted from Aspergillus nidulans
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DOI：
10.1248/bpb.28.1129
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发表时间：
2005-06-01
影响因子：
2
通讯作者：
Pombeiro-Sponchiado, SR
中科院分区：
文献类型：
--
作者：
Goncalves, RDR;Pombeiro-Sponchiado, SR
黑色素是由酚类或吲哚类化合物氧化聚合而成的高分子量色素。许多真菌，包括尼杜拉曲霉，会产生与黑色素相关或相同的色素，黑色素位于细胞壁上或以细胞外聚合物的形式存在。本研究的目的是评价合成黑色素和从黑曲霉高度黑化菌株(MEL1)生长后的菌丝体和培养基中提取的色素的抗氧化活性。通过不同浓度的黑色素对5-硫代-2-硝基苯甲酸(TNB)氧化的抑制，评价了黑色素对氧化剂HOCl和H_2O_2的清除能力。结果表明，MEL1菌株的色素与TNB竞争H_2O_2和HOCl，以浓度依赖的方式抑制TNB的氧化。对于HOCl氧化剂，这种抑制作用与合成黑色素相当，两者的IC50值相当接近。因此，我们的结果表明，海藻黑色素是一种潜在的HOCl清除剂，并可能被认为是化妆品行业的一种有前途的材料，用于配制保护皮肤免受可能的氧化损伤的面霜。
Melanins are pigments of high molecular weight formed by oxidative polymerization of phenolic or indolic compounds. A number of fungi, including Aspergillus nidulans, produce pigments related or identical to melanin, which are located on cell walls or exist as extracellular polymers. The aim of the present study was to assess the antioxidant activity of synthetic melanin and of the pigment extracted from the mycelium and culture medium after growth of the highly melanized strain (MEL1) from A. nidulans. The ability of the melanin pigment to scavenge the oxidants HOCl and H2O2 was evaluated by inhibition of the oxidation of 5-thio-2-nitrobenzoic acid (TNB) using several melanin concentrations. The results showed that the pigment of the MEL1 strain competes with TNB for H2O2 and HOCl, inhibiting TNB oxidation in a concentration-dependent manner. For the HOCl oxidant, this inhibition was comparable to that of synthetic melanin, whose IC50 values were quite close for both pigments. Thus, our results suggest that the melanin from A. nidulans is a potential HOCl scavenger and may be considered a promising material for the cosmetic industry for the formulation of creams that protect the skin against possible oxidative damage.