Distribution of O-glycosylhydrolases in Marine Fungi of the Sea of Japan and the Sea of Okhotsk: Characterization of Exocellular N-Acetyl-β-D-Glucosaminidase of the Marine Fungus Penicillium canescens
Distribution of O-glycosylhydrolases in Marine Fungi of the Sea of Japan and the Sea of Okhotsk: Characterization of Exocellular N-Acetyl-β-D-Glucosaminidase of the Marine Fungus Penicillium canescens
复制标题
DOI：
10.1134/s0003683810060141
复制
发表时间：
2010-11-01
影响因子：
0.8
通讯作者：
Zvyagintseva, T. N.
中科院分区：
文献类型：
--
作者：
Burtseva, Yu V.;Sova, V. V.;Zvyagintseva, T. N.
研究了来自鄂霍次克海（78 株）和日本海（14 株）不同海洋生境的 92 份真菌样品的胞外酶生产能力。发现了产生高活性聚糖酶和糖苷酶的菌株。通过向营养培养基中添加昆布多糖来刺激O-糖基水解酶的合成。从海洋真菌 Canescens 青霉中分离出高度纯化的 N-乙酰基-β-D-氨基葡萄糖苷酶。通过SDS-Na电泳测定酶的分子量为68 kDa。该酶在 pH 4.5 和温度 45°C 时表现出最大活性。酶在 50°C 下的半失活时间为 25 分钟。 N-乙酰基-β-D-氨基葡萄糖苷酶可水解β-氨基葡萄糖苷和β-氨基半乳糖苷键，并具有高转糖基活性。
The capacity to produce exocellular enzymes was studied for 92 samples of fungi from various marine habitats in the Sea of Okhotsk (78 strains) and the Sea of Japan (14 strains). Strains producing highly active glycanases and glycosidases were found. Synthesis of O-glycosylhydrolases was stimulated by addition of laminaran to the nutrient medium. Highly purified N-acetyl-beta-D-glucosaminidase was isolated from the marine fungus Penicillium canescens. The molecular weight of the enzyme determined by SDS-Na-electrophoresis was 68 kDa. The enzyme displayed maximum activity at pH 4.5 and temperature 45 degrees C. Inactivation half-time of the enzyme at 50 degrees C was 25 min. N-acetyl-beta-D-glucosaminidase hydrolyzed both beta-glucosaminide and beta-galactosaminide bonds and possessed a high transglycosylating activity.