Plasma metabolome analysis identifies distinct human metabotypes in the postprandial state with different susceptibility to weight loss-mediated metabolic improvements
Plasma metabolome analysis identifies distinct human metabotypes in the postprandial state with different susceptibility to weight loss-mediated metabolic improvements
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DOI：
10.1096/fj.201800330r
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发表时间：
2018-10-01
期刊：
影响因子：
4.8
通讯作者：
Daniel, Hannelore
中科院分区：
文献类型：
--
作者：
Fiamoncini, Jarlei;Rundle, Milena;Daniel, Hannelore
健康被定义为生物体适应挑战的能力。在这项研究中，我们测试了综合表型个体在多大程度上揭示了对标准化混合餐耐量试验（MMTT）的代谢反应差异，以及当个体经历中度体重减轻时这些反应如何变化。对70名健康人进行了代谢组学分析。在超过8小时的MMTT期间分析了大约300种血浆代谢物。脂肪酸代谢酶的血浆标志物的多变量分析鉴定了2个不同的代谢型簇（A和B）。与代谢型A的个体相比，代谢型B的个体显示葡萄糖清除较慢，腹腔内脂肪组织质量增加，肝脏脂质水平较高。基于核磁共振的尿液分析显示，这些人的饮食模式也不太健康。在12周内体重减轻约5.6 kg后，只有代谢型B的受试者在MMTT期间的血糖反应和代谢疾病标志物中显示出阳性变化。我们在健康个体中的研究表明，更全面的表型分型可以揭示在饮食干预中具有不同结果的离散代谢型，并且血浆中的脂质催化剂标志物可以允许代谢综合征的早期检测。Rundle，M.，Gibbons，H.，托马斯，E. L.，Geillinger-Kastle，K.，Bunzel，D.，Trezzi，J.- P.，Kiselova-Kaneva，Y.，Wopereis，S.，美国，Wahrheit，J.，Kulling，S. E、Hiller，K.，Sonntag，D.，Ivanova，D.，货车Ommen，B.，弗罗斯特，G.，布伦南，L.，丹尼尔，H.血浆代谢组学分析鉴定了餐后状态下对体重减轻介导的代谢改善具有不同易感性的不同人类代谢型。
Health has been defined as the capability of the organism to adapt to challenges. In this study, we tested to what extent comprehensively phenotyped individuals reveal differences in metabolic responses to a standardized mixed meal tolerance test (MMTT) and how these responses change when individuals experience moderate weight loss. Metabolome analysis was used in 70 healthy individuals. with profiling of approximate to 300 plasma metabolites during an MMTT over 8 h. Multivariate analysis of plasma markers of fatty acid catabolism identified 2 distinct metabotype clusters (A and B). Individuals from metabotype B showed slower glucose clearance, had increased intra-abdominal adipose tissue mass and higher hepatic lipid levels when compared with individuals from metabotype A. An NMR-based urine analysis revealed that these individuals also to have a less healthy dietary pattern. After a weight loss of approximate to 5.6 kg over 12 wk, only the subjects from metabotype B showed positive changes in the glycemic response during the MMTT and in markers of metabolic diseases. Our study in healthy individuals demonstrates that more comprehensive phenotyping can reveal discrete metabotypes with different outcomes in a dietary intervention and that markers of lipid catabolism in plasma could allow early detection of the metabolic syndrome.Fiamoncini, J., Rundle, M., Gibbons, H., Thomas, E. L., Geillinger-Kastle, K., Bunzel, D., Trezzi, J.-P., Kiselova-Kaneva, Y., Wopereis, S., Wahrheit, J., Kulling, S. E., Hiller, K., Sonntag, D., Ivanova, D., van Ommen, B., Frost, G., Brennan, L., Bell, J. Daniel, H. Plasma metabolome analysis identifies distinct human metabotypes in the postprandial state with different susceptibility to weight loss-mediated metabolic improvements.