Rat lung antioxidant enzyme induction by ozone.
Rat lung antioxidant enzyme induction by ozone.
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臭氧诱导大鼠肺抗氧化酶。
DOI：
10.1152/ajplung.1991.260.6.l412
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Massaro,D
中科院分区：
文献类型：
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作者：
Rahman,I;Clerch,LB;Massaro,D
我们将不同年龄的大鼠（体重约45-300 g）暴露于0.7 ppm O3中1-5天。在5天的O3暴露大鼠的肺部有较高的活性的铜，锌超氧化物歧化酶（SOD），锰SOD，过氧化氢酶，谷胱甘肽过氧化物酶比空气呼吸大鼠，这更大的活动是由于血液相关的酶活性。每种酶的mRNA浓度越高，酶活性越高（未测量Mn SOD），而这些mRNA的稳定性没有改变。在成年大鼠中，这些mRNA的浓度在暴露于O3 1，3和5天后进行测量，并在第3天升高。抗氧化酶（AOE）之间的组间差异（空气与O3）是不平等的，和组间差异的特定AOE mRNA的浓度大于其AOE活性的差异。我们得出结论，暴露于O3导致AOE基因的表达增加，表达的增加可能是在转录水平介导的。
We exposed rats of different ages (weights approximately 45-300 g) to 0.7 ppm O3 for 1-5 days. At 5 days lungs of O3-exposed rats had higher activity of Cu,Zn superoxide dismutase (SOD), Mn SOD, catalase, and glutathione peroxidase than air-breathing rats; this greater activity was not due to blood-associated enzyme activity. The greater enzyme activity occurred with a higher concentration of the mRNA for each enzyme (Mn SOD not measured) without altered stability of these mRNAs. In adult rats the concentrations of these mRNAs were measured after 1, 3, and 5 days exposure to O3 and were elevated by day 3. The intergroup differences (air vs. O3) among antioxidant enzymes (AOEs) were unequal, and the intergroup differences in concentration of the specific AOE mRNA were greater than the differences in activity of their AOE. We conclude exposure to O3 led to greater expression of AOE genes; the increased expression was mediated pretranslationally probably at the level of transcription.