The connective tissue framework in the normal prostate, BPH and prostate cancer: analysis by scanning electron microscopy after cellular digestion
The connective tissue framework in the normal prostate, BPH and prostate cancer: analysis by scanning electron microscopy after cellular digestion
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DOI：
10.1007/s002400000123
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发表时间：
2000-10-01
影响因子：
--
通讯作者：
Gaffney, E
中科院分区：
文献类型：
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作者：
Morrison, C;Thornhill, J;Gaffney, E
这项研究的目的有两个：（1）确定细胞消化过程是否可以促进前列腺结缔组织框架的形态检查，以及（2）检查正常前列腺组织、良性前列腺增生（BPH）和前列腺癌中的结缔组织框架。检查了十个前列腺。使用大谷消化法去除细胞成分。这使得扫描电子显微镜能够对前列腺组织内的结缔组织框架进行分析。组织块的光学显微镜确定了标本的组织学。前列腺由高度结构化的胶原纤维网络支撑。这种纤维网络在正常和患病状态下会有所不同。在良性前列腺增生中，胶原蛋白网络致密，纤维数量增加。在前列腺腺癌中，存在不均匀肿胀，并伴有胶原纤维的损失和分解。总之，氢氧化钠纤维素；消化提供了一种很好的方法来展示前列腺组织的结缔组织框架。正常前列腺、良性前列腺增生和前列腺腺癌中胶原纤维的形态变化对正常和患病状态下的前列腺生长具有影响。
The objective of this study was twofold: (1) to determine if a cellular digestion process can facilitate examination of the morphology of the connective tissue framework of the prostate, and (2) to examine the connective tissue framework in normal prostate tissue, benign prostatic hyperplasia (BPH) and prostate cancer. Ten prostate glands were examined. Using the Ohtani method of digestion, the cellular elements were removed. This enabled scanning electron microscopy analysis of the connective tissue framework within the prostatic tissue. Light microscopy of tissue blocks determined the histology of specimens. The prostate is supported by a highly structured network of collagen fibres. This network of fibres varies in normal and diseased states. In benign prostatic hyperplasia, the collagen network is dense, with an increased number of fibres. In prostatic adenocarcinoma, there is non-uniform swelling with a loss and disintegration of collagen fibres. In conclusion, sodium hydroxide cellula; digestion provides an excellent method for demonstrating the connective tissue framework of prostatic tissue. The morphological changes in collagen fibres in normal prostate, benign prostatic hyperplasia and prostatic adenocarcinoma have implications for prostate growth in normal and diseased states.