Expression of a transcriptional factor, SOX6, in human gliomas
Expression of a transcriptional factor, SOX6, in human gliomas
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DOI：
10.1007/bf02482175
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发表时间：
2004-07
影响因子：
3.3
通讯作者：
R. Ueda;Kazunari Yoshida;Y. Kawakami;T. Kawase;M. Toda
中科院分区：
文献类型：
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作者：
R. Ueda;Kazunari Yoshida;Y. Kawakami;T. Kawase;M. Toda
利用胶质瘤患者血清筛选人睾丸cDNA文库，分离出转录因子SOX6。在这里，我们使用免疫染色分析了SOX6在不同恶性级别胶质瘤中的表达。小鼠Sox6是一种转录因子，在发育中的中枢神经系统和小鼠胚胎软骨形成的早期阶段特异性表达。逆转录聚合酶链反应（RT-PCR）显示，SOX6基因在胶质瘤组织和胎儿脑中的表达高于正常成人脑组织和除黑色素瘤细胞外的其他癌细胞。抗SOX6抗体免疫组化分析显示，所有胶质瘤组织（胶质母细胞瘤14例，间变性星形细胞瘤14例，间变性少星形细胞瘤3例，弥漫性星形细胞瘤5例，少突胶质细胞瘤1例，毛细胞星形细胞瘤1例）在肿瘤细胞中均表达SOX6，而在大脑皮层非肿瘤组织中仅检测到少量SOX6阳性细胞。这些结果表明，发育调节转录因子SOX6可能是胶质瘤的潜在诊断标志物。
By screening a human testis cDNA library with glioma patients' sera, we isolated a transcriptional factor, SOX6. Here, we analyzed SOX6 expression in gliomas having a range of malignancy grades using immunostaining. Murine Sox6 is a transcriptional factor that is specifically expressed in the developing central nervous system and in the early stages of chondrogenesis in mouse embryos. The reverse transcription-polymerase chain reaction (RT-PCR) revealed that the SOX6 gene was more highly expressed in glioma tissues and fetal brain than in normal adult brain and other cancer cells, except melanoma cells. Immunohistochemical analysis with the anti-SOX6 antibody showed that all the glioma tissues analyzed (14 glioblastomas, 14 anaplastic astrocytomas, 3 anaplastic oligoastrocytomas, 5 diffuse astrocytomas, 1 oligodendroglioma, and 1 pilocytic astrocytoma) expressed SOX6 in tumor cells, but only a few SOX6-positive cells were detected in nonneoplastic tissues from the cerebral cortex. These results indicate that the developmentally regulated transcription factor SOX6 may be a potential diagnostic marker for gliomas.