Effect of Retinoid Status on the Messenger Ribonucleic Acid Expression of Nuclear Retinoid Receptors α, β, and γ, and Retinoid X Receptors α, β, and γ in the Mouse Testis.
Effect of Retinoid Status on the Messenger Ribonucleic Acid Expression of Nuclear Retinoid Receptors α, β, and γ, and Retinoid X Receptors α, β, and γ in the Mouse Testis.
复制标题
类维生素A状态对小鼠睾丸中核类维生素A受体α、β和γ以及类维生素A X受体α、β和γ的信使核糖核酸表达的影响。
DOI：
10.1210/endo.138.4.5051
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发表时间：
1997
期刊：
影响因子：
4.8
通讯作者：
D. D. de Rooij
中科院分区：
文献类型：
--
作者：
I. Gaemers;A. V. van Pelt;P. T. van der Saag;J. Hoogerbrugge;A. Themmen;D. D. de Rooij
在正常小鼠和维生素A缺乏小鼠中研究了全反式维甲酸（ATRA）给药后维甲酸受体（RAR α、RAR β和RAR γ）的睾丸基因表达。所有三种类型的RAR在正常和/或维生素A缺乏的睾丸中表达。注射ATRA后24 h内，RAR β mRNA仅被短暂诱导表达。在纯化的Sertoli细胞中也发现了ATRA诱导的RAR β表达，这表明这些细胞介导了类维生素A对生殖细胞的至少部分作用。当给予等摩尔量的视黄醇而不是ATRA时，在ATRA的最大诱导点没有观察到RAR β的诱导，这表明视黄醇的作用是延迟的，并且可能更少。相关的核受体RXR α，-β，以及第一次，γ，也被证明存在于小鼠睾丸中。ATRA给药后，RXR α和β的信使RNA表达没有显着变化。RXR γ的表达太低而无法定量。最后，检查了类维生素A代谢抑制剂利阿罗唑对ATRA诱导的A精原细胞增殖的影响。A精原细胞的标记指数，24小时后，0.25毫克的ATRA，显着降低由Liarozole由于最大的5-溴脱氧尿苷掺入到一个较早的点（20小时）的移动。这表明利阿唑延迟类维生素A代谢，从而增加实际ATRA浓度，更重要的是，ATRA本身是精子发生中的活性类维生素A。显然，ATRA不需要代谢为4-oxo-RA，这是以前被证明是一个更有效的诱导精原细胞增殖比ATRA，是有效的。
The testicular gene expression of the retinoic acid receptors, RAR alpha, -beta, and -gamma, was studied in normal mice and in vitamin A-deficient mice after the administration of all-trans-retinoic acid (ATRA). All three types of RARs were expressed in normal and/or vitamin A-deficient testes. Only the expression of RAR beta messenger RNA was transiently induced within 24 h after ATRA injection. ATRA-induced RAR beta expression was also found in purified Sertoli cells, suggesting that these cells mediate at least part of the effect of retinoids on germ cells. When an equimolar amount of retinol was administered instead of ATRA, no induction of RAR beta was seen at the point of maximal induction by ATRA, suggesting that the effect of retinol was delayed and probably less. The related nuclear receptors, RXR alpha, -beta, and, for the first time, gamma, were also shown to be present in the mouse testis. Upon administration of ATRA, messenger RNA expression of RXR alpha and -beta did not change significantly. The expression of RXR gamma was too low to allow quantification. Finally, the effect of the retinoid metabolism inhibitor liarozole on ATRA-induced proliferation of A spermatogonia was examined. The labeling index of A spermatogonia, 24 h after the administration of 0.25 mg ATRA, was significantly lowered by liarozole due to a shift of the maximal 5-bromo-deoxyuridine incorporation to an earlier point (20 h). This indicates that liarozole delays retinoid metabolism, thereby increasing the actual ATRA concentration, and more importantly, that ATRA by itself is an active retinoid in spermatogenesis. Apparently, ATRA does not need to be metabolized to 4-oxo-RA, which was previously shown to be a more potent inducer of spermatogonial proliferation than ATRA, to be effective.