Molecular Typing, Antibiogram and PCR-RFLP Based Detection of Aeromonas hydrophila Complex Isolated from Oreochromis niloticus
Molecular Typing, Antibiogram and PCR-RFLP Based Detection of Aeromonas hydrophila Complex Isolated from Oreochromis niloticus
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DOI：
10.3390/pathogens9030238
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发表时间：
2020-03-01
期刊：
影响因子：
3.7
通讯作者：
Mabrok, Mahmoud
中科院分区：
文献类型：
--
作者：
Algammal, Abdelazeem M.;Mohamed, Mohamed Fathi;Mabrok, Mahmoud
运动性气单胞菌败血症是尼罗罗非鱼常见的细菌性疾病，在世界范围内造成巨大的经济损失。为了解埃及尼罗罗非鱼气单胞菌复合群的流行情况、分子分型、图谱和生物多样性，从埃及El-Sharkia省的10个私人罗非鱼养殖场（25尾/场）随机采集了250尾尼罗罗非鱼。对收集的鱼进行临床和细菌学检查。大多数受感染的鱼表现出溃疡性坏死、眼球突出和出血性败血症的内部体征。A.水肿综合征占13.2%，其中肝脏是最主要的受累器官（54.1%）。采用聚合酶链反应（PCR）技术对A.使用一组靶向gyrB的引物以及毒力基因（aerA、alt和ahp）的检测来检测复合物。所有分离株均为gyrB保守基因阳性，并携带aerA和alt毒力基因。然而，这些分离株中没有一株ahp基因阳性。药敏试验结果表明，回收菌株对环丙沙星完全敏感，对阿莫西林高度耐药。所有检索到的菌株表现出相同的表型特征，并基于限制性片段长度多态性（RFLP）是相同的。实验攻毒鱼的死亡率较高（76.67%），表现出与自然感染鱼相似的典型症状。总之，表型和基因型特征的协同作用是一个有价值的流行病学工具，用于诊断A。- 是的RFLP是监测所有回收菌株生物多样性的基本工具。水蒸气
Motile Aeromonas septicemia is a common bacterial disease that affects Oreochromis niloticus and causes tremendous economic losses globally. In order to investigate the prevalence, molecular typing, antibiogram and the biodiversity of Aeromonas hydrophila complex, a total of 250 tilapia (Oreochromis niloticus) were collected randomly from 10 private tilapia farms (25 fish/farm) at El-Sharkia Governorate, Egypt. The collected fish were subjected to clinical and bacteriological examinations. The majority of infected fish displayed ulcerative necrosis, exophthalmia, and internal signs of hemorrhagic septicemia. The prevalence of A. hydrophia complex was 13.2%, where the liver was the most predominant affected organ (54.1%). Polymerase chain reaction (PCR) was used to verify the identification of A. hydrophila complex using one set of primers targeting gyrB as well as the detection of virulent genes (aerA, alt, and ahp). All isolates were positive for the gyrB-conserved gene and harbored aerA and alt virulence genes. However, none of those isolates were positive for the ahp gene. The antimicrobial sensitivity was carried out, where the recovered strains were completely sensitive to ciprofloxacin and highly resistant to amoxicillin. All retrieved strains showed the same phenotypic characteristics and were identical based on the restriction fragment length polymorphism (RFLP). Experimentally challenged fish presented a high mortality rate (76.67%) and showed typical signs as in naturally infected ones. In conclusion, the synergism of phenotypic and genotypic characterization is a valuable epidemiological tool for the diagnosis of A. hydrophila complex. RFLP is a fundamental tool for monitoring the biodiversity among all retrieved strains of A. hydrophia.