A cellular lineage analysis of the chick limb bud
A cellular lineage analysis of the chick limb bud
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DOI：
10.1016/j.ydbio.2007.08.002
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发表时间：
2007-10-15
影响因子：
2.7
通讯作者：
Tabin, C. J.
中科院分区：
文献类型：
--
作者：
Pearse, R. V., II;Scherz, P. J.;Tabin, C. J.
鸡肢芽已被用作研究模式形成和组织发育的模型系统超过50年。然而，肢体间充质内不同细胞类型之间的线性关系和单个细胞的迁移边界尚未被探索。我们已经使用了逆转录病毒谱系分析系统来跟踪单个肢芽间充质细胞在早期肢体发育的不同时间的命运。我们发现在第19-22阶段标记的祖细胞可以产生多种细胞类型，包括含有所有五种主要侧板中胚层衍生组织（软骨、软骨膜、肌腱、肌肉结缔组织和真皮）的细胞的克隆。然而，存在偏差，使得克隆更可能包含空间相邻组织的细胞类型，例如软骨/软骨膜和肌腱/肌肉结缔组织。最近有人提出，不同的proximodistal节建立在肢体发育的早期，然而，我们的分析表明，有没有严格的障碍，细胞迁移沿着proximodistal轴在早期阶段19-22肢芽。最后，我们的数据表明存在的背/腹边界建立的阶段16，是抑制细胞混合。该边界由LIM-同源结构域因子lmx 1b的表达划定。(C)2007爱思唯尔公司All rights reserved.
The chick limb bud has been used as a model system for studying pattern formation and tissue development for more than 50 years. However, the lineal relationships among the different cell types and the migrational boundaries of individual cells within the limb mesenchyme have not been explored. We have used a retroviral lineage analysis system to track the fate of single limb bud mesenchymal cells at different times in early limb development. We find that progenitor cells labeled at stage 19-22 can give rise to multiple cell types including clones containing cells of all five of the major lateral plate mesoderm-derived tissues (cartilage, perichondrium, tendon, muscle connective tissue, and dermis). There is a bias, however, such that clones are more likely to contain the cell types of spatially adjacent tissues such as cartilage/perichondrium and tendon/muscle connective tissue. It has been recently proposed that distinct proximodistal segments are established early in limb development; however our analysis suggests that there is not a strict barrier to cellular migration along the proximodistal axis in the early stage 19-22 limb buds. Finally, our data indicate the presence of a dorsal/ventral boundary established by stage 16 that is inhibitory to cellular mixing. This boundary is demarcated by the expression of the LIM-homeodomain factor lmx1b. (C) 2007 Elsevier Inc. All rights reserved.