Limited human-to-human transmission of avian influenza A(H7N9) virus, Shanghai, China, March to April 2013
Limited human-to-human transmission of avian influenza A(H7N9) virus, Shanghai, China, March to April 2013
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DOI：
10.2807/1560-7917.es2014.19.25.20838
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发表时间：
2014-06-26
期刊：
影响因子：
19
通讯作者：
Wu, F.
中科院分区：
文献类型：
--
作者：
Hu, J.;Zhu, Y.;Wu, F.
2013年4月，中国上海市一个家庭的两名成员相继被确诊为甲型H7N9禽流感病毒感染病例。采用实时逆转录（RT）-PCR检测了两例病例及其密切接触者的呼吸道标本。通过血凝抑制试验和微量中和试验检测接触者的配对血清标本。索引患者出现重度肺炎。她的丈夫在不久后患上了肺炎。这两个病例的临床特征高度相似，通过基因分析证实两个病例均感染了A（H7N9）病毒。系统发育分析表明，这两名患者的序列与闵行和长宁区湿货市场的环境样品之间具有高度的相似性。长宁菜市场6份样本确诊为甲型H7N9流感阳性。在27名密切接触者中，一人出现轻度呼吸道症状，另一人检测出A（H7N9）抗体阳性，但通过实时RT-PCR检测，两人均为阴性。两例病例的其他25名密切接触者均为甲型H7N9流感阴性。该病毒的有限人际传播最有可能发生在家庭集群中。然而，其他密切接触者的病毒检测结果未呈阳性，表明病毒在人际间广泛传播的可能性有限。
In April 2013, two members of one family were successively confirmed as cases of avian influenza A(H7N9) virus infection in Shanghai, China. Respiratory specimens from the two cases and their close contacts were tested using real-time reverse-transcription (RT)-PCR. Paired serum specimens from contacts were tested by haemagglutination inhibition assay and microneutralisation test. The index patient developed severe pneumonia. Her husband presented with pneumonia shortly thereafter. Both cases had highly similar clinical features and infection with A(H7N9) virus was confirmed in both cases by genetic analysis. Phylogenetic analysis revealed a high level of similarity between the sequences from the two patients and environmental samples collected from wet markets in Minhang and Changning districts. Six samples from the Changning wet market were confirmed as A(H7N9) positive. Of 27 close contacts, one developed mild respiratory symptoms and another tested positive for A(H7N9) antibodies, but both were negative by real-time RT-PCR. The other 25 close contacts of both cases were A(H7N9) negative. Limited human-to-human transmission of the virus most likely occurred in the family cluster. However, other close contacts did not test positive for the virus, suggesting limited potential for extensive human-to-human transmission of the virus.