Genes encoding alpha and beta subunits of Na,K-ATPase are located on three different chromosomes in the mouse.
Genes encoding alpha and beta subunits of Na,K-ATPase are located on three different chromosomes in the mouse.
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编码 Na,K-ATP 酶 α 和 β 亚基的基因位于小鼠的三个不同染色体上。
DOI：
10.1073/pnas.84.15.5369
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发表时间：
1987
影响因子：
11.1
通讯作者：
Housman,DE
中科院分区：
文献类型：
--
作者：
Kent,RB;Fallows,DA;Geissler,E;Glaser,T;Emanuel,JR;Lalley,PA;Levenson,R;Housman,DE
我们利用一组小鼠-仓鼠体细胞杂种和两种小鼠物种（小家鼠和小家鼠）之间的限制性片段长度多态性来确定编码Na，K-ATP酶（Na+，K+激活的ATP磷酸水解酶，EC 3.6.1.3）α和β亚基的基因的染色体定位。Na，K-ATP酶α亚基的三种不同亚型的DNA探针定位于三种不同的小鼠染色体：α 1基因（Atpa-1）与3号染色体上的Egf基因共分离; α 2（Atpa-2）与7号染色体上的细胞色素P-450 PB基因家族/香豆素羟化酶基因座共分离; α 3（Atpa-3）与1号染色体上的α-血影蛋白基因共分离。Na，K-ATP酶β亚基基因（Atpb）定位于1号染色体的同一区域，但与Atpa-3基因不紧密连锁。这些结果表明，Na，K-ATP酶α亚基的三种亚型由三个不同的基因编码。Na，K-ATP酶基因的分散表明它们的表达不太可能由共同的顺式作用调节元件控制。
We have made use of a panel of mouse-hamster somatic cell hybrids and restriction fragment length polymorphisms between two mouse species (Mus musculus and Mus spretus) to determine the chromosomal localization of genes encoding the alpha and beta subunits of the Na,K-ATPase (Na+,K+-activated ATP phosphohydrolase, EC 3.6.1.3). DNA probes for three distinct isoforms of the Na,K-ATPase alpha subunit mapped to three different mouse chromosomes: the alpha 1 gene (Atpa-1) cosegregated with the Egf gene on chromosome 3; alpha 2 (Atpa-2) with the cytochrome P-450PB gene family/coumarin hydroxylase locus on chromosome 7; alpha 3 (Atpa-3) with the alpha-spectrin gene on chromosome 1. The Na,K-ATPase beta-subunit gene (Atpb) mapped to the same region of chromosome 1, but it was not tightly linked to the Atpa-3 gene. These results indicate that three isoforms of the Na,K-ATPase alpha subunit are encoded by three distinct genes. The dispersion of Na,K-ATPase genes suggests that their expression is not likely to be controlled by a common cis-acting regulatory element.