Comparison of the phospholipid and triacylglycerol fatty acid profile of rat serum, skeletal muscle and heart
Comparison of the phospholipid and triacylglycerol fatty acid profile of rat serum, skeletal muscle and heart
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DOI：
10.33549/physiolres.930792
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发表时间：
2006-01-01
影响因子：
2.1
通讯作者：
Mougios, V.
中科院分区：
文献类型：
--
作者：
Nikolaidis, M. G.;Petridou, A.;Mougios, V.
虽然有几项研究同时分析了一种或多种组织中的磷脂（PL）和三酰甘油（TG）的脂肪酸谱，但缺乏对不同组织和/或脂类脂肪酸组成的系统比较。本研究的目的是比较大鼠血清、比目鱼肌、指长伸肌和心脏中主要脂类脂肪酸（PL和TG）的组成。从这些组织中提取脂质，并用薄层色谱和气相色谱相结合的方法进行分析。我们发现在各种组织和脂类中有许多显著的差异。血清在PL中具有最明显的脂肪酸谱，但这种“独特性”在TG中不太明显，其中组织之间的差异通常比PL更少。这两种骨骼肌在两种脂类中表现出相似的脂肪酸组成，尽管它们的肌纤维类型组成不同，这表明纤维类型不是大鼠骨骼肌脂肪酸组成的主要决定因素。心脏PL的脂肪酸谱与其他组织的差异最大。甘油三酯富含多不饱和脂肪酸，甘油三酯富含单不饱和脂肪酸。尽管在被检查的组织中脂肪酸分布差异的原因在很大程度上是未知的，但这些差异很可能对许多生物功能产生影响。
Although several studies have analyzed the fatty acid profile of phospholipids (PL) and, to a lesser degree, triacylglycerols (TG) in one or more tissues concurrently, a systematic comparison of the fatty acid composition of different tissues and/or lipid classes is lacking. The purpose of the present study was to compare the fatty acid composition of major lipid classes (PL and TG) in the rat serum, soleus muscle, extensor digitorum longus muscle and the heart. Lipids were extracted from these tissues and analyzed by a combination of thin-layer chromatography and gas chromatography. We found many significant differences in various tissues and lipid classes. Serum had the most distinct fatty acid profile in PL but this "uniqueness" was less apparent in TG, where differences among tissues were in general less frequent than in PL. These two skeletal muscles exhibited similar fatty acid composition in both lipid classes despite their different muscle fiber type composition, denoting that fiber type is not a major determinant of the fatty acid composition of rat skeletal muscle. The fatty acid profile of heart PL was the most different from that of the other tissues examined. PL were rich in polyunsaturated fatty acids, whereas TG were rich in monounsaturated fatty acids. Although the reasons for the differences in fatty acid profile among the tissues examined are largely unknown, it is likely that these differences have an impact upon numerous biological functions.