Surface functionalization of electrically conductive polypyrrole film with hyaluronic acid
Surface functionalization of electrically conductive polypyrrole film with hyaluronic acid
复制标题
DOI：
10.1021/la025979b
复制
发表时间：
2002-10-29
期刊：
影响因子：
3.9
通讯作者：
Kang, ET
中科院分区：
文献类型：
--
作者：
Cen, L;Neoh, KG;Kang, ET
用透明质酸(HA)对聚吡咯(PPY)导电薄膜进行表面改性。PPY薄膜首先与丙烯酸2-羟乙酯(HEA)接枝共聚，然后与(3-氨丙基)三乙氧基硅烷(ATS)通过Si-O键与接枝的HEA聚合物的羟基进行硅烷化。然后，通过ATS硅烷化过程，通过酰胺键的形成与PPY膜表面引入的伯胺基团共价固定了水溶性碳二亚胺活化的透明质酸。通过改变PPY膜表面伯胺基团的浓度，可以改变HA的固定量。蛋白质结合实验表明固定化HA具有生物活性，在空气中放置4天后仍保持较高的活性。将透明质酸共价固定在PPY薄膜表面，既能保持薄膜的导电性和机械强度，又能改善薄膜的表面润湿性。
A surface modification technique was employed to functionalize electrically conductive polypyrrole (PPY) films with hyaluronic acid (HA). The PPY film was first graft copolymerized with 2-hydroxyethyl acrylate (HEA) and subsequently silanized with (3-aminopropyl)triethoxysilane (ATS) via Si-O bonding with the hydroxyl groups of the grafted HEA polymer. Water-soluble carbodiimide-activated hyaluronic acid was then covalently immobilized through amide linkage formation with the primary amine groups introduced on the PPY film surface via the ATS silanization process. The amount of HA immobilized could be varied by changing the concentration of the primary amine groups on the PPY film surface. The immobilized HA was biologically active as evaluated by using a protein binding assay, and it retained a significant degree of its activity after 4 days storage in air. The present method of covalent immobilization of HA on PPY film surface offers the advantages of preserving the film's electrical conductivity and mechanical strength and at the same time improving the film's surface wettability.