The Treatment of Rhodiola Mimics Exercise to Resist High-Fat Diet-Induced Muscle Dysfunction via Sirtuin1-Dependent Mechanisms.
The Treatment of Rhodiola Mimics Exercise to Resist High-Fat Diet-Induced Muscle Dysfunction via Sirtuin1-Dependent Mechanisms.
复制标题
DOI：
10.3389/fphar.2021.646489
复制
发表时间：
2021
影响因子：
5.6
通讯作者：
Liu S
中科院分区：
文献类型：
--
作者：
You B;Dun Y;Fu S;Qi D;Zhang W;Liu Y;Qiu L;Xie M;Liu S
肌肉功能障碍是高脂饮食(HFD)诱导的肥胖的并发症，可以通过运动预防，但由于多种原因，患者通过运动未能获得足够的治疗效果。为了探索预防或治疗肥胖症患者肌肉功能障碍的替代或辅助方法，我们调查了红景天作为运动药片对肌肉功能障碍的影响。SIRT1可能抑制阿托金的表达，改善线粒体的质量控制，这可能是运动和红景天刺激的治疗靶点，但进一步的机制尚不清楚。我们验证了HFD喂养小鼠的脂代谢紊乱和骨骼肌功能障碍。此外，运动和红景天用于干预高血压病小鼠。我们的结果表明，运动和红景天可以预防肥胖小鼠肌肉萎缩和功能障碍，激活SIRT1途径，同时抑制萎缩基因，改善线粒体质量控制。用SIRT1抑制剂EX-527验证SIRT1在红景天苷获益中的重要作用。细胞培养实验结果表明，红景天苷可减轻高棕榈酸诱导的C2C12肌管萎缩和线粒体质量控制损伤，但EX-527可抑制红景天苷对C2C12肌管的上述作用。总体而言，红景天模拟激活SIRT1信号的运动，从而改善HFD诱导的肌肉功能障碍。
Muscle dysfunction is a complication of high-fat diet (HFD)-induced obesity that could be prevented by exercise, but patients did not get enough therapeutic efficacy from exercise due to multiple reasons. To explore alternative or supplementary approaches to prevent or treat muscle dysfunction in individuals with obesity, we investigated the effects of Rhodiola on muscle dysfunction as exercise pills. SIRT1 might suppress atrogenes expression and improve mitochondrial quality control, which could be a therapeutic target stimulated by exercise and Rhodiola, but further mechanisms remain unclear. We verified the lipid metabolism disorders and skeletal muscle dysfunction in HFD feeding mice. Moreover, exercise and Rhodiola were used to intervene mice with a HFD. Our results showed that exercise and Rhodiola prevented muscle atrophy and dysfunction in obese mice and activating the SIRT1 pathway, while atrogenes were suppressed and mitochondrial quality control was improved. EX-527, SIRT1 inhibitor, was used to validate the essential role of SIRT1 in salidroside benefit. Results of cell culture experiment showed that salidroside alleviated high palmitate-induced atrophy and mitochondrial quality control impairments, but these improvements of salidroside were inhibited by EX-527 in C2C12 myotubes. Overall, Rhodiola mimics exercise that activates SIRT1 signaling leading to improvement of HFD-induced muscle dysfunction.
登录
查看更多内容
DOI：
10.33594/000000282
发表时间：
2020-10-01
期刊：
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
影响因子：
--
作者：
Nisr, Raid B;Shah, Dinesh S;Hundal, Harinder S
通讯作者：
Hundal, Harinder S
影响因子：
9.3
作者：
Dun, Yaoshan;Thomas, Randal J.;Olson, Thomas P.
通讯作者：
Olson, Thomas P.
影响因子：
4.8
作者：
Lee, Donghoon;Goldberg, Alfred L.
通讯作者：
Goldberg, Alfred L.
影响因子：
11
作者：
Lei, Yun;Wang, Jiangong;Lu, Xin-Yun
通讯作者：
Lu, Xin-Yun
靶向Sirtuin 1以改善新陈代谢：您所需要的是NAD（+）？
影响因子：
21.1
作者：
Cantó C;Auwerx J
通讯作者：
Auwerx J