Neurite outgrowth inhibition by chondroitin sulfate proteoglycan: Stalling/stopping exceeds turning in human neuroblastoma growth cones
Neurite outgrowth inhibition by chondroitin sulfate proteoglycan: Stalling/stopping exceeds turning in human neuroblastoma growth cones
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DOI：
10.1006/exnr.1999.7212
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发表时间：
1999-11-01
影响因子：
5.3
通讯作者：
Snow, DM
中科院分区：
文献类型：
--
作者：
Hynds, DL;Snow, DM
硫酸软骨素蛋白聚糖（CSPG）在体内和体内抑制来自鸡胚和啮齿类动物神经元的生长，并且在发育期间和损伤后上调。CSPG在来自人类神经元的生长中的作用在很大程度上未经测试，但对于我们理解人类神经系统损伤后的再生至关重要。在这里，我们确定了CSPG对血小板衍生生长因子（PDGF）刺激的SH-SY 5 Y人神经母细胞瘤细胞（一种公认的神经元分化模型）的神经突生长的影响。将细胞接种在吸附有层粘连蛋白（LN）、CSPG或由两种分子的交替条纹组成的图案化基质的盖玻片上。与其他使用鸡或啮齿动物神经元的研究类似，SH-SY 5 Y细胞在LN上延伸神经突，与平板接种在玻璃上的细胞相比，显示出总神经突长度/细胞增加15.2%。与接种在玻璃或LN上的细胞相比，接种在单独CSPG上的细胞表现出减少的神经突生长。有趣的是，SH-SY 5 Y生长锥在LN上延伸，然后遇到CSPG：边界，显示更多的停止/停滞（62.3%）比转向（27.9%）行为。可溶性CSPG：抑制接种在玻璃上的SH-SY 5 Y细胞的神经突起始，但不抑制LN。这些数据表明，人神经元样细胞的几种CSPG引起的反应与非人神经元的反应相似。然而，大约70%的SH-SY 5 Y生长锥在CSPG边界处破裂或停滞，而超过80%的鸡感觉神经元在CSPG边界处转向。这些模型之间的实验差异很可能表明动物和人类神经元再生之间的功能差异。(C)北京：科学出版社.
Chondroitin sulfate proteoglycan (CSPG) inhibits outgrowth from embryonic chick and rodent neurons in vivo and in vivo and is upregulated during development and following injury, The role of CSPG; in outgrowth from human neurons has been largely untested, but is critical for our understanding of regeneration in humans following nervous system injury. Here we determined the effects of CSPG on platelet-derived growth factor (PDGF)-stimulated neurite outgrowth from SH-SY5Y human neuroblastoma cells, a well-accepted model of neuronal differentiation. Cells were plated on glass coverslips adsorbed with laminin (LN), CSPG, or a patterned substratum consisting of alternating stripes of the two molecules. Similar to other studies using chick or rodent neurons, SH-SY5Y cells extend neurites on LN, displaying a 15.2% increase in the total neurite length/cell as compared to cells plated on glass. Cells plated on CSPG: alone exhibited reduced neurite outgrowth compared to cells plated on glass or LN. interestingly, SH-SY5Y growth cones extending on LN and then encountering a CSPG: border display more stopping/stalling (62.3%) than turning (27.9%) behaviors. Soluble CSPG: inhibits neurite initiation from SH-SY5Y cells plated on glass, but not on LN. These data demonstrate that several CSPG-elicited responses of human neuron-like cells are similar to those from nonhuman neurons. However, approximately 70% of SH-SY5Y growth cones sfop or stall at a CSPG border while over 80% of chick sensory neurons turn at a CSPG border. The experimental difference between these models may well indicate a functional difference between animal and human neuronal regeneration. (C) 1999 Academic Press.