Comparison of open and closed methods for vitrification of human embryos and the elimination of potential contamination
Comparison of open and closed methods for vitrification of human embryos and the elimination of potential contamination
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DOI：
10.1016/s1472-6483(10)61169-8
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发表时间：
2005-11-01
影响因子：
4
通讯作者：
Kato, O
中科院分区：
文献类型：
--
作者：
Kuwayama, M;Vajta, G;Kato, O
在缓慢冷却与玻璃化冷冻后，人类胚胎的存活和发育进行了比较，涉及超过13，000个玻璃化冷冻胚胎。此外，使用人胚泡比较了开放系统Cryotop和封闭玻璃化冷冻系统CryoTip（TM）的功效。玻璃化冷冻的人原核期胚胎100%存活，52%发育为囊胚，而缓慢冷却后的存活率为89%，囊胚发育率为41%。与缓慢冷却（91%）相比，玻璃化冷冻4细胞胚胎（98%）的存活率相似。此外，90%的玻璃化冷冻囊胚存活，并导致53%的妊娠率转移后，相比之下，84%的存活率和51%的妊娠率缓慢冷却。所有相应值均存在显著差异。当比较封闭式和开放式玻璃化冷冻系统时，在支持囊胚存活率（CryoTip和Cryotop分别为93%和97%）、妊娠率（分别为51%和59%）和分娩率（分别为48%和51%）方面没有发现差异。玻璃化冷冻是一种简单，有效和具有成本效益的方法，可以提高每个周期的累积妊娠率。封闭式CryoTip系统的使用消除了冷冻保存和储存期间胚胎污染的可能性，而不会影响体外和体内的存活率和发育率。
Survival and development of human embryos was compared following slow cooling versus vitrification involving more than 13,000 vitrified embryos. In addition, the efficacy of an open system, the Cryotop, and a closed vitrification system, the CryoTip(TM), were compared using human blastocysts. One hundred percent of vitrified human pronuclear stage embryos survived and 52% developed to blastocysts as compared with 89% survival and 41% blastocyst development after slow cooling. Similar survival rates were seen with vitrification of 4-cell embryos (98%) as compared with slow cooling (91%). Furthermore, 90% of vitrified blastocysts survived and resulted in a 53% pregnancy rate following transfer, as compared with 84% survival and 51% pregnancy rates following slow cooling. All corresponding values were significantly different. When the closed and open vitrification systems were compared, no difference was found with regard to supporting blastocyst survival (93 and 97% for CryoTip and Cryotop respectively), pregnancies (51 versus 59% respectively) and deliveries (48 versus 51% respectively). Vitrification is a simple, efficient and cost-effective way to improve cumulative pregnancy rates per cycle. The use of the closed CryoTip system eliminates the potential for embryo contamination during cryopreservation and storage without compromising survival and developmental rates in vitro and in vivo.