Inheritance of T-associated sex reversal in mice.
Inheritance of T-associated sex reversal in mice.
复制标题
小鼠中 T 相关性逆转的遗传。
DOI：
10.1017/s001667230003528x
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Eicher,EM
中科院分区：
文献类型：
--
作者：
Washburn,LL;Lee,BK;Eicher,EM
我们之前在小鼠Chr 17上发现了一个主要的性别决定基因Tas，如果存在AKR/J Y染色体，该基因会导致C57BL/6J THP/+和Torl/+个体的卵巢组织发育。根据THP位于C57BL/6J近交系背景下XY小鼠卵巢组织发育的观察，我们推测Tas位于THP和Torl缺失的Chr17区域内，C57BL/6J携带诊断Tas等位基因，而当THP位于C3H/HeSnJ近交系背景下时，XY小鼠卵巢组织发育正常。为了验证这一假设，我们将(C57BL/6J×C3H/HeSnJ)F1雌鼠与C57BL/6J THP/+两性人交配。正如预期的那样，XY THP/+后代中有一半发育了卵巢和睾丸组织，而另一半只发育了睾丸组织。出乎意料的是，所选择的Chr17分子座位的遗传与性腺发育无关，因为一半的雄性后代和两性后代遗传了C3H/HeSnJ来源的Chr17基因座，一半遗传了C57BL/6J来源的Chr17基因座。我们的结论是，卵巢组织要在XY THP/+或XY Tort/+个体中发育：(1)Tas必须处于半合子状态，这是通过THP或Tort缺失的杂合性实现的；(2)必须存在AKR/J来源的Y染色体；(3)参与初级性别决定的另一个基因座必须处于纯合子C57BL/6J状态。这个新发现的基因可能是先前定义的TDA-1或TDA-2基因座之一。
We previously identified a primary sex-determining locus, Tas, on mouse Chr 17 that causes ovarian tissue development in C57BL/6J Thp/ + and Torl/ + individuals if the AKR/J Y chromosome is present. We hypothesized that Tas is located within the region of Chr 17 deleted by Thp and Torl and that C57BL/6J carries a diagnostic Tas allele, based on the observation that ovarian tissue develops in XY mice when Thp is on a C57BL/6J inbred strain background, whereas normal testicular development occurs when Thp is on a C3H/HeSnJ inbred strain background. To test this hypothesis, we mated (C57BL/6J × C3H/HeSnJ)F1 females to C57BL/6J Thp/ + hermaphrodites. As expected, half of the XY Thp /+ offspring developed ovarian and testicular tissue while half developed exclusively testicular tissue. Unexpectedly, the inheritance of selected Chr 17 molecular loci was independent of gonadal development, as half of the male and hermaphroditic offspring inherited C3H/HeSnJ-derived Chr 17 loci and half inherited C57BL/6J-derived Chr 17 loci. We conclude that for ovarian tissue to develop in an XY Thp/ + or XY Tort/ + individual (1) Tas must be present in a hemizygous state, which is accomplished by heterozygosity for the Thp or Tort deletions; (2) the AKR/J-derived Y chromosome must be present; and (3) an additional locus involved in primary sex determination must be present in a homozygous C57BL/6J state. This newly identified gene may be one of the previously defined loci, tda-1 or tda-2.