Sizes of components in frog skeletal muscle measured by methods of stereology.
Sizes of components in frog skeletal muscle measured by methods of stereology.
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DOI：
10.1085/jgp.66.1.31
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发表时间：
1975-07
期刊：
影响因子：
--
通讯作者：
Eisenberg BR
中科院分区：
文献类型：
--
作者：
Mobley BA;Eisenberg BR
应用点计数和交点计数的体视学方法，对青蛙骨骼肌的光镜和电镜照片进行了形态学参数的测量。缝匠肌的结果如下：肌原纤维占纤维体积的83%，它们的表面与体积比为3.8妈妈-1。线粒体占纤维体积的1.6%。横小管占纤维体积的0.32%，每体积纤维的表面积为0.22 μ m-1。肌浆网的末端池包括4.1%的纤维体积，其表面积每体积的纤维是0.54妈妈-1。纵向肌浆网占纤维体积的5.0%，每体积纤维的表面积为1.48 μ m-1。纵向桥梁之间的终端池两侧的Z盘很少观察到，他们只占0.035%的纤维体积和每体积的纤维的表面积为0.009妈妈-1。T-SR连接出现在横小管表面的67%以上和终池表面的27%以上。小窝的表面体积比为48妈妈-1;小窝可增加肌膜表面积的47%。从半腱肌纤维获得基本相同的结果。
Stereological techniques of point and intersection counting were used to measure morphological parameters from light and electron micrographs of frog skeletal muscle. Results for sartorius muscle are as follows: myofibrils comprise 83% of fiber volume; their surface to volume ratio is 3.8 mum-1. Mitochondria comprise 1.6% of fiber volume. Transverse tubules comprise 0.32% of fiber volume, and their surface area per volume of fiber is 0.22 mum-1. Terminal cisternae of the sarcoplasmic reticulum comprise 4.1% of fiber volume; their surface area per volume of fiber is 0.54 mum-1. Longitudinal sarcoplasmic reticullum comprises 5.0% of fiber volume, and its surface area per volume of fiber is 1.48 mum-1. Longitudinal bridges between terminal cisternae on either side of a Z disk were observed infrequently; they make up only 0.035% of fiber volume and their surface area per volume of fiber is 0.009 mum-1. T-SR junction occurs over 67% of the surface of transverse tubules and over 27% of the surface of terminal cisternae. The surface to volume ratio of the caveolae is 48 mum-1; caveolae may increase the sarcolemmal surface area by 47%. Essentially the same results were obtained from semitendinosus fibers.