CONSEQUENCES OF SPATIAL SEPARATION OF PHOTOSYSTEM-1 AND PHOTOSYSTEM-2 IN THYLAKOID MEMBRANES OF HIGHER-PLANT CHLOROPLASTS
CONSEQUENCES OF SPATIAL SEPARATION OF PHOTOSYSTEM-1 AND PHOTOSYSTEM-2 IN THYLAKOID MEMBRANES OF HIGHER-PLANT CHLOROPLASTS
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DOI：
10.1016/0014-5793(81)80041-5
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发表时间：
1981-01-01
期刊：
影响因子：
3.5
通讯作者：
ANDERSON, JM
中科院分区：
文献类型：
--
作者：
ANDERSON, JM
大多数高等植物和一些绿藻的类囊体膜在结构上组织成紧密接触的贴贴膜网络，即颗粒类囊体膜，它们与单个的、未堆叠的膜，即基质类囊体膜相互连接[1 -5]。这些类囊体膜的内表面在颗粒和间质类囊体之间包围了一个连续的空间。如图1所示，类囊体有两个不同的膜区，这里称为暴露膜和贴载膜。外露的类胶体，其外表面与基质直接接触，包括基质类胶体和颗粒堆的端部和边缘。相反，颗粒隔膜的外表面与基质的接触受到限制。冻裂电子显微镜显示了附着膜和暴露膜中冻裂颗粒的大小、形状和密度的差异[5-81]。这反映了两个区域类囊体膜的主要内在大分子复合物分布的差异。类囊体的这种引人注目的结构组织与功能的分化是平行的。用洗涤剂[9]或机械方法将~类囊体分离成颗粒和基质类囊体组分[j]，表明大的亚氯-
The thylakoid membranes of most higher plants and some green algae are structurally organized into a network of closely contacting, appressed membranes, the grana thylakoids, which are interconnected with single, unstacked membranes, the stroma thylakoids [l-5]. The inner surface of these thylakoid membranes encloses a space which is continuous between the grana and stroma thylakoids. As shown schematically in fig. 1, thylakoids have two distinct membrane regions, termed here exposed and appressed membranes. The exposed~ ylakoids whose outer surfaces are in direct contact with the stroma, include stroma~ ylakoids and the end membr~ es and margins of the grana stacks. In contrast, the outer surfaces of the appressed membranes of the grana partitions have limited access to the stroma. Freeze-fracture electron microscopy reveals a difference in the size, shape and density of freeze-fracture particles located in appressed and exposed membranes [5-81. This reflects a difference in the distribution of the main intrinsic macromolecular complexes of thylakoid membranes in the two regions. This striking structural organization of thylakoids is paralleled by a differentiation of function. Fractionation of~ ylakoids into grana and stroma thylakoid fractions by detergent [9] or mechanical methods [IO], shows that the large subchlo-