Malathion-Induced Oxidative Stress in Human Erythrocytes and the Protective Effect of Vitamins C and E In Vitro
Malathion-Induced Oxidative Stress in Human Erythrocytes and the Protective Effect of Vitamins C and E In Vitro
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DOI：
10.1002/tox.20423
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发表时间：
2009-06-01
影响因子：
4.5
通讯作者：
Kalender, Yusuf
中科院分区：
文献类型：
--
作者：
Durak, Dilek;Uzun, Fatma Goekce;Kalender, Yusuf
马拉硫磷是一种有机磷（OP）农药，已被证明可以通过产生自由基和改变细胞抗氧化防御系统来诱导红细胞中的氧化应激。我们研究了几种不同剂量的马拉硫磷（25，75，200亩M），或马拉硫磷与维生素C（VC; 10亩M）或维生素E（VE; 30亩M）的组合，对丙二醛（MDA）的水平，超氧化物歧化酶（SOD），过氧化氢酶（CAT），谷胱甘肽过氧化物酶（GPx）的活性在体外人红细胞。红细胞在各种处理条件（单独马拉硫磷、单独维生素或马拉硫磷加维生素）下于37 ℃孵育60分钟，并测定MDA水平以及SOD、CAT和GPx活性。单独使用马拉硫磷可使红细胞MDA含量升高，SOD、CAT和GPx活性降低（P < 0.05）。与未处理的对照细胞相比，VC处理的、VE处理的或VC + VE处理的红细胞之间没有统计学差异。用马拉硫磷+ VC、马拉硫磷+ VE或所有三种药剂的组合处理细胞防止了马拉硫磷诱导的抗氧化酶活性和脂质过氧化的变化。然而，这种效果只出现在低浓度的马拉硫磷（25和75 μ M），和VC + VE的组合有一个更好的保护效果比单独的VC或VE。这些结果表明，维生素的浓度与血浆中发现的水平相似，在通常用于杀虫剂的马拉硫磷浓度（200 μ M）下，维生素的存在对马拉硫磷诱导的红细胞毒性没有影响。(C)2008 Wiley Periodcals，Inc.环境毒理学24：235-242，2009年。
Malathion is an organophosphate (OP) pesticide that has been shown to induce oxidative stress in erythrocytes through the generation of free radicals and alteration of the cellular antioxidant defense system. We examined the effect of several different doses of malathion (25, 75, 200 mu M), or malathion in combination with vitamin C (VC; 10 mu M) or vitamin E (VE; 30 mu M), on the levels of malondialdehyde (MDA), and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities in human erythrocytes in vitro. Erythrocytes were incubated under various treatment conditions (malathion alone, vitamins alone, or malathion plus vitamin) at 37 degrees C for 60 min, and the levels of MDA, and SOD, CAT and GPx activities, were determined. Treatment with malathion alone increased the levels of MDA and decreased SOD, CAT, and GPx activities in erythrocytes (P < 0.05). There were no statistical differences among VC-treated, VE-treated, or VC + VE-treated erythrocyes, as compared with nontreated control cells. Treatment of cells with malathion + VC, malathion + VE, or a combination of all three agents prevented malathion-induced changes in antioxidant enzyme activity and lipid peroxidation. However, this effect was seen only at low concentrations of malathion (25 and 75 mu M), and the combination of VC + VE had a more protective effect than VC or VE alone. These results indicated that the presence of vitamins at concentrations that are similar to the levels found in plasma have no effect on malathion-induced toxicity in erythrocytes at a concentration of malathion (200 mu M) that is typically used in pesticides. (C) 2008 Wiley Periodcals, Inc. Environ Toxicol 24: 235-242, 2009.