Detection of Escherichia coli O157:H7 using a surface plasmon resonance biosensor
Detection of Escherichia coli O157:H7 using a surface plasmon resonance biosensor
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DOI：
10.1023/a:1008872002336
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发表时间：
1998-07-01
影响因子：
--
通讯作者：
Gehring, AG
中科院分区：
文献类型：
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作者：
Fratamico, PM;Strobaugh, TP;Gehring, AG
用Biacore生物传感器检测大肠杆菌O157：H7与特异性抗体的结合。固定的蛋白A或蛋白G捕获抗体，然后抗体与细菌结合。或者，固定化抗体捕获了大肠杆菌O157：H7，并通过第二种抗体进一步探测细菌，从而增强了信号。再生的传感器表面被用于至少50个单独的分析。表面等离子体共振生物传感器在细菌的快速、实时检测和鉴定以及研究生物与不同抗血清或其他分子物种的相互作用方面具有潜在的应用前景。
The BIAcore biosensor was used to detect binding of Escherichia coli O157:H7 with specific antibodies. Immobilized Protein A or Protein G captured antibodies which in turn bound to the bacteria. Alternatively, immobilized antibody captured the E. coli O157:H7 and the bacteria were further probed by a second antibody which enhanced the signal. The regenerated sensor surfaces were used for at least 50 separate analyses. The surface plasmon resonance biosensor has potential for use in rapid, real-time detection and identification of bacteria, and to study the interaction of organisms with different antisera or other molecular species.