High-Temperature Stress Alleviation by Selenium Nanoparticle Treatment in Grain Sorghum
High-Temperature Stress Alleviation by Selenium Nanoparticle Treatment in Grain Sorghum
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DOI：
10.1021/acsomega.7b01934
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发表时间：
2018-03-01
期刊：
影响因子：
4.1
通讯作者：
Prasad, P. V. Vara
中科院分区：
文献类型：
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作者：
Djanaguiraman, M.;Belliraj, N.;Prasad, P. V. Vara
硒纳米颗粒（Se-NPs）在缓解作物高温胁迫中的作用尚不清楚。研究了高温胁迫下高粱对硒- nps的吸收、毒性及生理生物学效应。Moench]。合成了尺寸为10 ~ 40 nm的Se-NPs，并对其进行了表征。毒性试验显示，硒- nps浓度为275 mg L-1，致50%细胞死亡。易位研究表明，Se-NPs可以从高粱根部向茎部转移。高温胁迫下高粱孕穗期叶面喷施10 mg L-1 Se-NPs，通过提高抗氧化酶活性刺激抗氧化防御系统。此外，它还降低了特征氧化剂的浓度。硒- nps促进了不饱和磷脂水平的提高。高温胁迫下Se-NPs提高了花粉发芽率，显著提高了种子产量。高温（38/28℃）条件下抗氧化酶活性的增加和抗氧化剂含量的减少明显大于最适温度（32/22℃）条件下。综上所述，Se-NPs可以通过增强抗氧化防御系统来保护高粱植株免受高温胁迫。
The role of selenium nanoparticles (Se-NPs) in the mitigation of high-temperature (HT) stress in crops is not known. The uptake, toxicity and physiological and biological effects of Se-NPs under HT were investigated in grain sorghum [Sorghum bicolor (L.) Moench]. Se-NPs of size 10-40 nm were synthesized and characterized to indicate nanocrystalline structure. A toxicity assay showed that Se-NPs concentration inducing 50% cell mortality (TC50) was 275 mg L-1. Translocation study indicated that Se-NPs can move from root to shoot of sorghum plants. Foliar spray of 10 mg L-1 Se-NPs during the booting stage of sorghum grown under HT stress stimulated the antioxidant defense system by enhancing antioxidant enzymes activity. Furthermore, it decreased the concentration of signature oxidants. Se-NPs facilitated higher levels of unsaturated phospholipids. Se-NPs under HT stress improved the pollen germination percentage, leading to a significantly increased seed yield. The increased antioxidant enzyme activity and decreased content of oxidants in the presence of Se-NPs were greater under HT (38/28 degrees C) than under optimum temperature conditions (32/22 degrees C). In conclusion, Se-NPs can protect sorghum plants by enhanced antioxidative defense system under HT stress.