An early transient decrease in phosphatidylinositol 4,5-bisphosphate upon stimulation of rabbit platelets with acetylglycerylether phosphorylcholine (platelet activating factor).
An early transient decrease in phosphatidylinositol 4,5-bisphosphate upon stimulation of rabbit platelets with acetylglycerylether phosphorylcholine (platelet activating factor).
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用乙酰甘油醚磷酸胆碱（血小板激活因子）刺激兔血小板后，磷脂酰肌醇 4,5-二磷酸早期短暂减少。
DOI：
10.1016/0003-9861(83)90492-7
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发表时间：
1983
影响因子：
3.9
通讯作者：
Hanahan,DJ
中科院分区：
文献类型：
--
作者：
Shukla,SD;Hanahan,DJ
用AGEPC（1-O-烷基-2-乙酰基-sn-甘油基-3-磷酸胆碱）（5 × 10− 10 μ m）刺激用[3 H]肌醇标记的洗涤过的兔血小板不同时间。在这些血小板与AGEPC混合后5 s内，[3 H]TPI（磷脂酰肌醇4，5-二磷酸）明显降低约25%;此后TPI的放射性立即升高。用不同浓度的AGEPC处理这些标记血小板仅5秒，表明[3 H]TPI呈特征性剂量相关性降低。在AGEPC诱导的血小板刺激后，磷脂酰肌醇4-磷酸的放射性也出现增加。有趣的是，在15秒内，观察到[3 H]PI（磷脂酰肌醇）降低15 - 20%，[3 H]lysoPI增加。然而，[3 H]lysoPI可能仅与[3 H]PI降低的三分之一相关。LysoGEPC（溶血-1-O-烷基-sn-甘油基-3-磷酸胆碱）对血小板活化无效，不能引起磷酸肌醇的任何变化。TPI的状态以时间和剂量依赖性方式受到影响以及这些变化发生的速度表明，这种肌醇磷脂可能与伴随AGEPC与血小板相互作用的早期过程密切相关。
Washed rabbit platelets labeled with [3H]inositol were stimulated with AGEPC (1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine) (5 × 10−10m) for various time periods. Within 5 s of the mixing of these platelets with AGEPC, an approximately 25% decrease in the [3H]TPI (phosphatidylinositol 4,5-bisphosphate) was evident; immediately thereafter the radioactivity in TPI increased. These labeled platelets treated with various concentrations of AGEPC for only 5 s indicated a characteristic dose-related decrease in [3H]TPI. Radioactivity in phosphatidylinositol 4-phosphate also appeared to increase after AGEPC-induced stimulation of platelets. Interestingly, within 15 s a 15 to 20% decrease in [3H]PI (phosphatidylinositol) and an increase in [3H]lysoPI was observed. However, [3H]lysoPI could be related only to one-third of the decrease in [3H]PI. LysoGEPC (lyso-1-O-alkyl-sn-glyceryl-3-phosphorylcholine), which is ineffective in the activation of platelets, was unable to cause any changes in the phosphoinositides. The fact that the status of TPI was influenced in a time- and dose-dependent manner and the rapidity with which these changes take place suggest that this inositol phospholipid may be associated closely with the early processes which accompany the interaction of AGEPC with platelets.