PCR characterization and typing of Klebsiella pneumoniae using capsular type-specific, variable number tandem repeat and virulence gene targets
PCR characterization and typing of Klebsiella pneumoniae using capsular type-specific, variable number tandem repeat and virulence gene targets
复制标题
DOI：
10.1099/jmm.0.015198-0
复制
发表时间：
2010-05-01
影响因子：
3
通讯作者：
Hampton, Catherine V.
中科院分区：
文献类型：
--
作者：
Turton, Jane F.;Perry, Claire;Hampton, Catherine V.
描述了一种多重PCR，其检测与侵袭性疾病或致病性最相关的荚膜类型K1、K2、K5、K54和K57、另一种荚膜类型（K20）、两种推定的毒力因子（rmpA和wcaG）和肺炎克雷伯氏菌的16 S-23 S内部转录间隔区单元，以便于鉴定该微生物。wcaG编码被膜岩藻糖的产生，并与被膜类型K1和K54相关，但也在其他被膜类型的菌株中发现，筛选的543个分离株中有18个对该基因呈PCR阳性。设计了一个八位点可变数目串联重复序列（VNTR）方案，该方案在一组代表29个PFGE类型的36个分离物中提供了与PFGE提供的相似水平的区分。所有与化脓性肝脓肿相关的CC 23强毒K1克隆的测试分离物具有相同的VNTR谱，这可能有助于鉴定该克隆，这些方法提供了一种快速鉴定和分型这种重要的社区获得性和医院感染病原体的方法。
A multiplex PCR is described which detects capsular types K1, K2, K5, K54 and K57, which are those most associated with invasive disease or pathogenicity, a further capsular type (K20), two putative virulence factors (rmpA and wcaG) and the 16S-23S internal transcribed spacer unit of Klebsiella pneumoniae, facilitating identification of this organism. wcaG encodes capsular fucose production and was associated with capsular types K1 and K54, but was also found in strains of other capsular types, 18 of the 543 isolates screened were PCR-positive for this gene. An eight-locus variable number tandem repeat (VNTR) scheme was designed, which provided discrimination at a level similar to that afforded by PFGE among a panel of 36 isolates representing 29 PFGE types All isolates tested of the virulent K1 clone of CC23, associated with pyogenic liver abscesses, shared the same VNTR profile, which may be helpful in identifying this clone, such isolates were also PCR-positive for allS These methods provide a rapid means of characterizing and typing isolates of this important agent of community-acquired and nosocomial infection