Reciprocal Genetics: Identifying QTL for General and Specific Combining Abilities in Hybrids Between Multiparental Populations from Two Maize (Zea mays L.) Heterotic Groups.
Reciprocal Genetics: Identifying QTL for General and Specific Combining Abilities in Hybrids Between Multiparental Populations from Two Maize (Zea mays L.) Heterotic Groups.
复制标题
DOI：
10.1534/genetics.117.300305
复制
发表时间：
2017-11
期刊：
影响因子：
3.3
通讯作者：
Moreau L
中科院分区：
文献类型：
--
作者：
Giraud H;Bauland C;Falque M;Madur D;Combes V;Jamin P;Monteil C;Laborde J;Palaffre C;Gaillard A;Blanchard P;Charcosset A;Moreau L
了解杂种性能的遗传结构对于显示杂种优势的物种具有重要意义。Giraud等人。评估和..。一些具有农业重要性的动植物物种被商业化为杂交品种，以利用杂种优势现象。因此，了解杂交性能的遗传结构是至关重要的。我们育成了两个多亲本玉米(Zea Mays L.)群体，每个群体对应于一个重要的杂种优势群(凹陷或火石)，并由4个方正品系发出的6个相连的自交系双亲分离群体(每个群体分别为802和822个系)组成。不使用“测试者”来评估它们的杂交价值，而是根据不完全析因设计杂交分离系，产生951个锯齿火石杂交种，在8个环境中对4个生物产量性状进行评估。对杂交值的一般配合力(GCA)和特殊配合力(SCA)成分进行了QTL检测，同时考虑了各方正系传递的等位基因效应。共检测到42个QTL。检测到主要影响一般配合力的QTL，其中31%(干物质产量)对特殊配合力的影响较小。显性效应的小影响与已知的凹陷和火石杂种群体之间的分化以及由于我们设计中观察到的特殊配合力(不同性状的∼为20%)而产生的杂交方差的小百分比一致。此外，大多数(80%)的一般配合力QTL只在两个杂种优势群中的一个群中分离。与基于测试器的设计相比，利用两个多亲群体之间的杂交来同时检测两个杂种群体中的QTL似乎具有很高的成本效益。这为利用分子标记选育优良杂交组合提供了新的前景。
Understanding genetic architecture of hybrid performances is important for species showing heterosis. Giraud et al. evaluated an... Several plant and animal species of agricultural importance are commercialized as hybrids to take advantage of the heterosis phenomenon. Understanding the genetic architecture of hybrid performances is therefore of key importance. We developed two multiparental maize (Zea mays L.) populations, each corresponding to an important heterotic group (dent or flint) and comprised of six connected biparental segregating populations of inbred lines (802 and 822 lines for each group, respectively) issued from four founder lines. Instead of using “testers” to evaluate their hybrid values, segregating lines were crossed according to an incomplete factorial design to produce 951 dent–flint hybrids, evaluated for four biomass production traits in eight environments. QTL detection was carried out for the general-combining-ability (GCA) and specific-combining-ability (SCA) components of hybrid value, considering allelic effects transmitted from each founder line. In total, 42 QTL were detected across traits. We detected mostly QTL affecting GCA, 31% (41% for dry matter yield) of which also had mild effects on SCA. The small impact of dominant effects is consistent with the known differentiation between the dent and flint heterotic groups and the small percentage of hybrid variance due to SCA observed in our design (∼20% for the different traits). Furthermore, most (80%) of GCA QTL were segregating in only one of the two heterotic groups. Relative to tester-based designs, use of hybrids between two multiparental populations appears highly cost efficient to detect QTL in two heterotic groups simultaneously. This presents new prospects for selecting superior hybrid combinations with markers.