The Effect of the Implantation of Spirometra mansonoides Spargana in the Growing Rat
The Effect of the Implantation of Spirometra mansonoides Spargana in the Growing Rat
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三棱螺植入生长大鼠体内的效果
DOI：
10.3181/00379727-138-35952
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发表时间：
1971
影响因子：
--
通讯作者：
S. Steelman
中科院分区：
文献类型：
--
作者：
M. Glitzer;S. Steelman
Mueller和Reed(1)报告说，在正常生长的大鼠体内植入曼陀罗并不会增加体重。这与他们的发现相反，正常小鼠、仓鼠、甲状腺功能减退和垂体切除的大鼠植入了雀形目动物，体重显著增加。斯蒂尔曼等人。(2)研究表明，成年高原雌性大鼠对植入裂头虫的体重增加的反应与生长激素产生的体重增加相似。由于生长激素和裂头盖骨生长因子(SGF)的生物学特性非常相似，因此有必要对裂头盖植入物对正常生长的雄性和雌性大鼠的影响进行更详细的研究。初步实验表明，植入裂头雀的动物的内源性生长激素分泌可能会发生改变。方法：研究方法。卡沃斯农场的雄性和雌性大鼠的平均体重在100-160g之间。在任何一个实验中，动物都根据禁食的体重被分成不同的组，使得平均组体重相同，平均体重的标准误差相同。所有动物都被随意喂食实验室标准饲料颗粒和自来水，并在恒温和光照条件下被单独饲养在笼子里。用剪下的大约1厘米长的裂头植入(2)，悬吊在生理盐水中，用18号针的注射器将其注射到颈部背侧。每只动物身上都植入了10个头骨。对照组动物没有接受任何植入。在最初的研究中，15只雄性和雌性大鼠被植入裂片，并保持了14天。
Mueller and Reed (1) have reported that the implantation of S. mansonoides spargana in normally growing rats did not enhance body weight gain. This was in contrast to their findings of marked weight gain in normal mice, hamsters, and hypothyroid and hypophysectomized rats implanted with spargana. Steelman et al. (2) have shown that the adult plateaued female rat responds to spargana implants with a body weight increase similar to that produced by growth hormone. Due to the close similarities of the biological profiles of growth hormone and sparganum growth factor (SGF), it was of interest to conduct a more detailed examination of the effects of spargana implants in intact growing male and female rats. Preliminary experiments suggested that endogenous growth hormone secretion may be modified in spargana-implanted animals. Methods. Groups of male and female rats (Carworth Farms), whose average body weights were from 100 to 160 g, were employed in the studies. In any one experiment, animals were allocated into groups on the basis of fasted body weight in such a manner that the average group body weights were the same and the standard errors of the mean body weights were comparable. All animals were fed standard laboratory chow pellets and tap water ad libitum, and were maintained in individual cages under constant temperature and lighting conditions. Clipped scolices (approx 1 cm in length) of spargana were used for implantation (2) and were suspended in physiological saline and injected subcutaneously into the dorsal side of the neck using a syringe with an 18 gauge needle. Ten scolices were implanted in each animal. The control animals received no implants. In the initial study, groups of 15 male and female rats were implanted with spargana and were maintained for 14 days.