ASYMMETRIC LEAVES2-LIKE15 gene, a member of AS2/LOB family, shows a dual abaxializing or adaxializing function in Arabidopsis lateral organs
ASYMMETRIC LEAVES2-LIKE15 gene, a member of AS2/LOB family, shows a dual abaxializing or adaxializing function in Arabidopsis lateral organs
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ASYMMETRIC LEAVES2-LIKE15 基因是 AS2/LOB 家族的成员，在拟南芥侧器官中显示出双重远轴化或近轴化功能
DOI：
10.1007/s11738-016-2256-x
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发表时间：
2016-10-01
影响因子：
2.6
通讯作者：
Jiang, Ji-Hong
中科院分区：
文献类型：
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作者：
Meng, Lai-Sheng;Wang, Zhi-Bo;Jiang, Ji-Hong
拟南芥ASYMMERTIC LEAVES 1（AS 1）和AS 2/LBD 6基因在决定叶片近轴命运中起重要作用。拟南芥不对称叶2-LIKE 15/LBD 17基因是LATERAL ORGAN BOUNDARY DOMAIN基因家族的成员之一，在幼年侧根的基部以及分生组织和器官原基之间的边界表达。为了更好地了解ASL 15/LBD 17在拟南芥发育中的作用，构建了ASL 15/LBD 17反义表达载体，获得了反义表达突变体35 S：ASL 15/LBD 17转基因植株。转基因植物可以在不同形状的莲座叶中指定远轴或近轴细胞命运。与Col-0相比，稍窄和极窄的莲座丛叶（I类）表现出远轴细胞性状。这些转基因植株的花序茎也表现出远轴器官的同一性。径向针状和丝状对称莲座叶（II类）提出的近轴细胞的命运，与野生型（Col-0）。然而，在AS 2突变的背景下，反义35 S：ASL 15仅表现出远轴器官的同一性，这意味着反义35：ASL 15的影响需要功能性AS 2基因。此外，反义35 S：ASL 15在近远侧模式中是缺陷的。此外，反义35 S：ASL 15导致KNOX基因转录水平的增强，即，KNAT 2、KNAT 6和STM，但不包括相关的KNAT 1基因。综上所述，我们的研究结果表明，ASL 15/LBD 17具有双重abaxializing或adaxializing功能，也与拟南芥侧器官的远端和近端图案。因此，ASL 15/LBD 17在拟南芥发育中具有多种功能。
In determining leaf adaxial fates, Arabidopsis ASYMMERTIC LEAVES1 (AS1) and AS2/LBD6 are essential. The Arabidopsis ASYMMETRIC LEAVES2-LIKE15/LBD17 gene, one member of the LATERAL ORGAN BOUNDARY DOMAIN gene family, is expressed at the base of juvenile lateral roots, as well as in boundaries between meristems and organ primordial. For better comprehending the function of ASL15/LBD17 in the Arabidopsis development, its antisense-expression construct was constructed, and some 35S:ASL15/LBD17 transgenic plants that were antisense overexpression mutants were attained. The transgenic plants can specify either abaxial or adaxial cell fate in differently shaped rosette leaves. In detail, slightly and extremely narrow rosette leaves (class I) displayed abaxial cell traits, compared with those of Col-0. Inflorescence stems of these transgenic plants also showed abaxial organ identity. Both radial needle-like and filamentous symmetric rosette leaves (class II) presented adaxial cell fate, in contrast to those of wild type (Col-0). However, in the background of AS2 mutation, antisense 35S: ASL15 only presented abaxial organ identity, implying that the influences of antisense 35: ASL15 require a functional AS2 gene. In addition, antisense 35S: ASL15 was defective in proximodistal patterning. In addition, antisense 35S: ASL15 lead to the enhancement of the transcript levels of the KNOX genes, i.e., KNAT2, KNAT6, and STM, but not of the related KNAT1 gene. Taken together, our findings show that ASL15/LBD17 has a dual abaxializing or adaxializing function, and is also related to distal and proximal patterning in the lateral organs of Arabidopsis. Therefore, ASL15/LBD17 has multiple functions in the Arabidopsis development.