Highly Repeatable Dissolution Dynamic Nuclear Polarization for Heteronuclear NMR Metabolomics
Highly Repeatable Dissolution Dynamic Nuclear Polarization for Heteronuclear NMR Metabolomics
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DOI：
10.1021/acs.analchem.6b01094
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发表时间：
2016-06-21
影响因子：
7.4
通讯作者：
Giraudeau, Patrick
中科院分区：
文献类型：
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作者：
Bornet, Aurelien;Maucourt, Mickael;Giraudeau, Patrick
在天然的C-13丰度下，基于异核磁共振的代谢组学受到敏感性的限制。我们最近证明了在交叉极化（CP）的辅助下，通过溶解动态核极化（D-DNP）的超极化提供了一种可靠的方法来提高异核磁共振在稀释代谢物混合物中的灵敏度。在本技术说明中，我们评估了这种实验方法的精度，这是代谢组学应用的关键点。重复性越高，就越有可能发现样本之间微小的生物学相关差异。我们最先进的D-DNP NMR设备对代谢组学相关样品（20 mg干重番茄提取物）的平均重复性在定量限（LOQ）以上的信号为3.6%，在考虑检测限（LOD）以上的所有信号时为6.4%。这是关于D-DNP重复性的第一份报告，强调了该技术与代谢组学要求的兼容性，并证实了其作为此类应用的分析工具的潜力。
At natural C-13 abundance, metabolomics based on heteronuclear NMR is limited by sensitivity. We have recently demonstrated how hyperpolarization by dissolution dynamic nuclear polarization (D-DNP) assisted by cross-polarization (CP) provides a reliable way of enhancing the sensitivity of heteronudear NMR in dilute mixtures of metabolites. In this Technical Note, we evaluate the precision of this experimental approach, a critical point for applications to metabolomics. The higher the repeatability, the greater the likelihood that one can detect small biologically relevant differences between samples. The average repeatability of our state-of-the-art D-DNP NMR equipment for samples of metabolomic relevance (20 mg dry weight tomato extracts) is 3.6% for signals above the limit of quantification (LOQ) and 6.4% when all the signals above the limit of detection (LOD) are taken into account. This first report on the repeatability of D-DNP highlights the compatibility of the technique with the requirements of metabolomics and confirms its potential as an analytical tool for such applications.