Rate maintenance of cell division in Escherichia coli B/r: analysis of a simple nutritional shift-down.
Rate maintenance of cell division in Escherichia coli B/r: analysis of a simple nutritional shift-down.
复制标题
大肠杆菌 B/r 中细胞分裂的速率维持：简单营养转变的分析。
DOI：
10.1128/jb.174.24.8152-8155.1992
复制
发表时间：
1992
影响因子：
3.2
通讯作者：
Helmstetter,CE
中科院分区：
文献类型：
--
作者：
Zaritsky,A;Helmstetter,CE
葡萄糖摄取的竞争性（不可代谢）抑制剂α-甲基葡萄糖苷用于限制大肠杆菌的生长。在分批培养中并使用“婴儿机器”技术研究了这种营养转变过程中的细胞分裂。经过短暂的延迟后，分批培养中的分裂速率维持 60 至 70 分钟，而在婴儿机器中则维持较长一段时间。细胞大小的减小部分是由于复制起始时每个染色体起点的质量可能减少以及起始和随后的分裂之间的时间间隔较短。这些不寻常的发现表明，这种在不改变抑制模式的情况下突然改变生长速率的方法对于研究细菌细胞各个方面的控制很有用。
A competitive (nonmetabolizable) inhibitor of glucose uptake, alpha-methylglucoside, was used to limit the growth of Escherichia coli. Cell division during such a nutritional shift-down was studied in batch cultures and with the "baby-machine" technique. Following a brief delay, the rate of division was maintained for 60 to 70 min in batch cultures and for an extended period in the baby machine. Decreases in cell size were due, in part, to a possible reduction in the mass per chromosome origin at the time of replication initiation and a shorter time interval between initiation and the subsequent division. These unusual findings suggest that this method for abrupt change in growth rate without modifying repression patterns is useful for studying the control of various aspects of the bacterial cell.