The Genome of Medicinal Plant Macleaya cordata Provides New Insights into Benzylisoquinoline Alkaloids Metabolism
The Genome of Medicinal Plant Macleaya cordata Provides New Insights into Benzylisoquinoline Alkaloids Metabolism
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药用植物博落回基因组为苄基异喹啉生物碱代谢提供新见解
DOI：
10.1016/j.molp.2017.05.007
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发表时间：
2017-07-05
期刊：
影响因子：
27.5
通讯作者：
Zeng, Jianguo
中科院分区：
文献类型：
--
作者：
Liu, Xiubin;Liu, Yisong;Zeng, Jianguo
抗生素在畜牧、农业和医药领域的过度使用对公众健康造成了一系列潜在威胁。博落回为罂粟科药用植物，为家畜抗菌饲料添加剂的生产提供了安全的资源。紫草中的活性成分已知包括苄基异喹啉生物碱(BIAS)，如血根碱(SAN)和白屈菜红碱(CHE)，但它们的代谢途径尚未在这种非模式植物中进行研究。通过摄食13C标记的酪氨酸，首次检测和证实了心形分枝杆菌中SAN和ChE的活性合成。为了获得进一步的洞察，我们重新测序了心毛分枝杆菌的整个基因组，这是第一个从罂粟科植物中测序出来的。心型支原体基因组全长378 Mb，编码22,328个蛋白质编码基因，其中43.5%为转座元件。作为基础优生根的一员，紫云英基因组缺少几乎所有优生根都发生的古六倍体事件。从基因组学数据中检索到与SAN和ChE生物合成相关的16个代谢基因，并验证了它们的14个生化活性。这些基因组学和代谢数据揭示了黑曲霉中保守的BIA代谢途径，为今后通过作物改良或微生物途径重建来生产SAN和ChE提供了知识基础。
The overuse of antibiotics in animal agriculture and medicine has caused a series of potential threats to public health. Macleaya cordata is a medicinal plant species from the Papaveraceae family, providing a safe resource for the manufacture of antimicrobial feed additive for livestock. The active constituents from M. cordata are known to include benzyliso-quinoline alkaloids (BIAs) such as sanguinarine (SAN) and chelerythrine (CHE), but their metabolic pathways have yet to be studied in this non-model plant. The active biosynthesis of SAN and CHE in M. cordata was first examined and confirmed by feeding 13 C-labeled tyrosine. To gain further insights, we de novo sequenced the whole genome of M. cordata, the first to be sequenced from the Papaveraceae family. The M. cordata genome covering 378 Mb encodes 22,328 predicted protein-coding genes with 43.5% being transposable elements. As a member of basal eudicot, M. cordata genome lacks the paleohexaploidy event that occurred in almost all eudicots. From the genomics data, a complete set of 16 metabolic genes for SAN and CHE biosynthesis was retrieved, and 14 of their biochemical activities were validated. These genomics and metabolic data show the conserved BIA metabolic pathways in M. cordata and provide the knowledge foundation for future productions of SAN and CHE by crop improvement or microbial pathway reconstruction.