Bleomycin delivery into cancer cells in vitro with ultrasound and SonoVue® or BR14® microbubbles
Bleomycin delivery into cancer cells in vitro with ultrasound and SonoVue® or BR14® microbubbles
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DOI：
10.3109/1061186x.2012.761223
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发表时间：
2013-05-01
影响因子：
4.5
通讯作者：
Bouakaz, A.
中科院分区：
文献类型：
--
作者：
Lamanauskas, N.;Novell, A.;Bouakaz, A.
工作背景：在存在造影剂微泡（MB）的情况下，细胞暴露于超声（US）可导致细胞声致穿孔，其可用于药物或基因递送。抗癌药物博莱霉素（BLM）用于声孔技术，在体内外均能有效地清除肿瘤细胞。然而，声致穿孔机制是未知的，因此使用不同的US参数和MB类型。最近，我们提出细胞声致孔的效率可能与MB声致破坏的效率有关。目的：我们分析了人肿瘤细胞对BLM、US和MB处理的反应。方法：人胶质母细胞瘤星形细胞瘤（U-87 MG）或结肠癌（HCT-116）细胞在BLM和SonoVue（R）或BR 14（R）MB存在下暴露于US。结果：超声辐照后HCT-116和U-87 MG细胞活力均下降30%。结论：声致孔介导BLM进入多种肿瘤细胞系是一种可行的方法。细胞声致孔效率与MB声破坏相关，提供了通过测量MB声破坏优化US参数的可能性。
Background: Cell exposure to ultrasound (US) in the presence of contrast agent microbubbles (MBs) can result in cell sonoporation that can be exploited for drug or gene delivery. Anticancer drug bleomycin (BLM), used in sonoporation, can effectively eliminate tumor cells in vitro and in vivo. Nevertheless, sonoporation mechanism is not known, thus different US parameters and MB types are used. Recently, we proposed that efficiency of cell sonoporation can be related to the efficiency of MB sonodestruction.Purpose: We analyzed human tumor cells viability in response to BLM, US and MB treatment.Methods: Human glioblastoma astrocytoma (U-87 MG) or colon cancer (HCT-116) cells were exposed to US in the presence of BLM and either SonoVue(R) or BR14(R) MBs. MB sonodestruction was evaluated according to US signal attenuation.Results: Both HCT-116 and U-87 MG cell viability following US exposure decreased up to 30%. Decrease in cell viability followed similar tendency as MB sonodestruction, which suggests direct relationship between MB sonodestruction and BLM intracellular delivery.Conclusion: Sonoporation is a feasible method to deliver BLM in to several types of human cancer cell lines. Efficiency of cell sonoporation correlated well with MB sonodestruction, providing a possibility to optimize US parameters by measuring MB sonodestruction.