Ionic mechanism of morphological changes of cultured myocardial cells on successive incubation in media without and with Ca2+.
Ionic mechanism of morphological changes of cultured myocardial cells on successive incubation in media without and with Ca2+.
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培养的心肌细胞在不含和含 Ca2 的培养基中连续培养后形态变化的离子机制。
DOI：
10.1016/0022-2828(80)90062-0
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发表时间：
1980
影响因子：
5
通讯作者：
A. Masuda
中科院分区：
文献类型：
--
作者：
K. Goshima;S. Wakabayashi;A. Masuda
(1)培养的小鼠心肌细胞在无Ca 2+培养基中短暂预孵育后在含Ca 2+的培养基中孵育引起形态学变化，如肌原纤维的完全收缩和细胞膜的气球形成（“Ca 2+悖论现象”）。(2)当心肌细胞在无Ca ~（2+）的培养液中预孵育后，再在含不同浓度Ca ~（2+）的培养液中孵育时，随着Ca ~（2+）浓度的增加，出现形态学改变的细胞百分比和Ca ~（2+）摄取率均增加。(3)在含有10− 7 mCa 2+或更少的培养基中预孵育对于在含有Ca 2+的培养基中孵育期间诱导形态变化和过量摄取Ca 2+是必要的。(4)当心肌细胞在含有不同浓度Na+的无Ca ~（2+）培养液中预孵育，再在含有Ca ~（2+）的培养液中孵育时，随着预孵育液中Na+浓度的增加，出现形态学改变的细胞百分比和Ca ~（2+）摄取率均增加。(5)各种治疗，抑制过量摄取的Ca 2+的细胞抑制形态学变化的发生。因此，所观察到的心肌细胞的形态学变化是由于心肌细胞过量摄取Ca 2+。(6)当心肌细胞在含有不同浓度Ca ~（2+）和固定浓度Na ~+的培养液中孵育时，细胞内Na ~+浓度随培养液中Ca ~（2+）浓度的降低而增加。预先加载了较高浓度Na+的细胞吸收Ca 2+的速度更快。(7)在不含Ca 2+的预孵育期间抑制细胞摄入Na+的条件会抑制在随后与Ca 2+一起孵育期间细胞摄入Ca 2+。这些结果表明，心肌细胞在培养基中与Ca ~（2+）孵育期间的Ca ~（2+）摄取依赖于细胞内Na ~+浓度。
(1) Incubation of cultured mouse myocardial cells in medium containing Ca2+after brief pre-incubation in Ca2+-free medium caused morphological changes, such as full contraction of myofibrils and balloon formation of the cell membrane (“Ca2+paradox phenomenon”). (2) When myocardial cells were pre-incubated in Ca2+-free medium, and then incubated in medium containing various concentrations of Ca2+, both the percentage of cells showing morphological changes and the rate of Ca2+uptake increased with increase in the Ca2+concentration. (3) Pre-incubation in medium containing 10−7mCa2+or less was necessary for induction of both morphological changes and excess uptake of Ca2+during incubation in medium containing Ca2+. (4) When myocardial cells were pre-incubated in Ca2+-free medium containing various concentrations of Na+, and then incubated in medium containing Ca2+, both the percentage of cells showing morphological changes and the rate of Ca2+uptake increased with increase in the Na+concentration in the pre-incubation medium. (5) Various treatments that inhibited excess uptake of Ca2+by the cells inhibited the genesis of morphological changes. Thus the observed morphological changes of myocardial cells were due to excess uptake of Ca2+by the myocardial cells. (6) When myocardial cells were incubated in medium containing various concentrations of Ca2+and a fixed concentration of Na+, the intracellular concentration of Na+increased with decrease in the Ca2+concentration of the medium. Cells that had been preloaded with a higher concentration of Na+took up the Ca2+faster. (7) Conditions that inhibited Na+uptake by the cells during pre-incubation without Ca2+inhibited Ca2+uptake by the cells during subsequent incubation with Ca2+. These results suggest that Ca2+uptake by myocardial cells during incubation in medium with Ca2+depends upon the intracellular Na+concentration.