THE REACTION OF ARACHIDONIC-ACID EPOXIDES (EPOXYEICOSATRIENOIC ACIDS) WITH A CYTOSOLIC EPOXIDE HYDROLASE
THE REACTION OF ARACHIDONIC-ACID EPOXIDES (EPOXYEICOSATRIENOIC ACIDS) WITH A CYTOSOLIC EPOXIDE HYDROLASE
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DOI：
10.1016/0003-9861(83)90628-8
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发表时间：
1983-01-01
影响因子：
3.9
通讯作者：
ESTABROOK, RW
中科院分区：
文献类型：
--
作者：
CHACOS, N;CAPDEVILA, J;ESTABROOK, RW
环氧二十碳三烯酸是在[大鼠]细胞色素P-450催化的花生四烯酸氧化过程中形成的，它与[小鼠]肝细胞浆中的环氧化物水解酶反应生成邻位二十碳三烯酸二醇。这种胞浆酶的作用，而不是微粒体结合型，解释了以前的结果表明，在体外有氧稳定状态下，肝微粒体氧化代谢花生四烯酸的能力积累环氧化物。根据最近有关5，6-环氧二十碳三烯酸可能的独特生理功能的研究，讨论了它不能作为该酶的合适底物的原因。
Epoxyeicosatrienoic acids, formed during the [rat] cytochrome P-450-catalyzed oxidation of arachidonic acid, react with a [mouse] liver cytosolic epoxide hydrolase to form vicinal diols of eicosatrienoic acid. The role of this cytosolic enzyme, rather than a microsomal bound type, explains previous results illustrating the ability to accumulate epoxides during the in vitro aerobic steady state of oxidative metabolism of arachidonic acid by liver microsomes. The inability of the 5,6-epoxyeicosatrienoic acid to serve as a suitable substrate for this enzyme is discussed in light of recent studies concerning possible unique physiological functions for this metabolite.