Evolutionary motif and its biological and structural significance
Evolutionary motif and its biological and structural significance
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DOI：
10.1007/pl00000056
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发表时间：
1997-01-01
影响因子：
3.9
通讯作者：
Gojobori, T
中科院分区：
文献类型：
--
作者：
Tateno, Y;Ikeo, K;Gojobori, T
我们开发了一种用于多重比对蛋白质序列的方法。该方法的主要特征是，它将所讨论的蛋白质的进化关系重复考虑以进行执行，直到关系和一致性结果一致为止。然后，我们将此方法应用于国际DNA序列数据库的数据，该数据库是世界上最全面，最新的DNA数据库，以通过广泛使用超级计算机来估计“进化主题”。尽管需要解决一些问题，但我们可以估计基序的长度在20至200个氨基酸范围内，最常见的长度约为60。然后，我们讨论了它们的生物学和结构意义。我们认为，我们现在可以分析DNA和蛋白质不仅在体内和体外，而且可以在计算机中进行分析。
We developed a method for multiple alignment of protein sequences. The main feature of this method is that it takes the evolutionary relationships of the proteins in question into account repeatedly for execution, until the relationships and alignment results are in agreement. We then applied this method to the data of the international DNA sequence databases, which are the most comprehensive and updated DNA databases in the world, in order to estimate the ''evolutionary motif'' by extensive use of a supercomputer. Though a few problems needed to be solved, we could estimate the length of the motifs in the range of 20 to 200 amino acids, with about 60 the most frequent length. We then discussed their biological and structural significance. We believe that we are now in a position to analyze DNA and protein not only in vivo and in vitro but also in silico.