Hydatidiform mole macromolecules inhibit interleukin-2-mediated murine lymphocyte proliferation in vitro.
Hydatidiform mole macromolecules inhibit interleukin-2-mediated murine lymphocyte proliferation in vitro.
复制标题
葡萄胎大分子在体外抑制白细胞介素2介导的小鼠淋巴细胞增殖。
DOI：
10.1111/j.1600-0897.1988.tb00239.x
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Cowan,BD
中科院分区：
文献类型：
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作者：
Bennett,WA;Ellsaesser,CF;Cowan,BD
从葡萄胎滋养层提取的大分子抑制有丝分裂原诱导的淋巴细胞增殖。为了表征这种免疫调节的机制，我们测定了葡萄胎囊液（HMF）和组织提取物（HME）对体外淋巴因子功能的影响。分别使用淋巴瘤细胞系（LBRM-33 - 1A 5）和鼠T细胞系（CTLL 2）测定白细胞介素-1（IL-1）和白细胞介素-2（IL-2）的利用率。HMF在500（对照组的36.4%）和50（对照组的74.9%）μg/ml时抑制（P <0.05）IL-2依赖性CTLL 2细胞增殖。HME还在500（对照的46.0%）、100（对照的67.2%）、50（对照的71.5%）和10（对照的85.4%）μg/培养物ml下抑制CTLL 2增殖（P <0.05）。相比之下，HMF对IL-1刺激的LBRM-33 - 1A 5产生IL-2没有影响。然而，在IL-1利用测定中，500 μg/ml HME抑制（P <0.05）IL-2产生（对照的63.0%）。这种抑制作用可能是由于HME从LBRM-33 - 1A 5培养物携带到用于测量IL-2产生的靶细胞（CTLL 2）。HME样品的分子量色谱洗脱低分子量（35-50 kd）和高分子量（> 250 kd）级分中的IL-2抑制剂。这些数据表明，来自葡萄胎的大分子可能干扰体外免疫应答的一种方式是调节白细胞介素-2的功能。
Macromolecules extracted from hydatidiform mole trophoblast inhibit mitogen‐induced lymphocyte proliferation. To characterize the mechanism of this immunomodulation, we determined the effects of hydatidiform mole vesicle fluid (HMF) and tissue extracts (HME) on lymphokine function in vitro. Utilization of interleukin‐1 (IL‐1) and interleukin‐2 (IL‐2) were determined by using a lymphoma cell line (LBRM‐33‐1A5) and a murine T cell line (CTLL2), respectively. HMF suppressed (P < .05) IL‐2‐dependent CTLL2 cell proliferation at 500 (36.4% of controls) and 50 (74.9% of controls) μg/ml. HME also suppressed CTLL2 proliferation (P < .05) at 500 (46.0% of controls), 100 (67.2% of controls), 50 (71.5% of controls), and 10 (85.4% of controls) μg/culture ml. In contrast, HMF exhibited no effect on IL‐1‐stimulated LBRM‐33‐1A5 production of IL‐2. However, 500 μg/ml of HME inhibited (P < .05) IL‐2 production (63.0% of controls) in the IL‐1 utilization assay. This suppressive effect was probably due to a carry over of HME from the LBRM‐33‐1A5 culture to the target cells (CTLL2) used to measure IL‐2 production. Molecular weight chromatography of an HME sample eluted an IL‐2 inhibitor in a low molecular weight (35–50 kd) and high molecular weight (> 250 kd) fraction. These data suggest that one way in which macromolecules derived from hydatidiform mole could interfere with in vitro immunologic responses is by modulating interleukin‐2 function.