OsIDD2, a zinc finger and INDETERMINATE DOMAIN protein, regulates secondary cell wall formation
OsIDD2, a zinc finger and INDETERMINATE DOMAIN protein, regulates secondary cell wall formation
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DOI：
10.1111/jipb.12557
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发表时间：
2018-02-01
影响因子：
11.4
通讯作者：
Ueguchi-Tanaka, Miyako
中科院分区：
文献类型：
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作者：
Huang, Peng;Yoshida, Hideki;Ueguchi-Tanaka, Miyako
在此之前，我们发现123个转录因子（TF）的候选调控次生细胞壁（SCW）的形成在水稻中使用系统发育和共表达网络分析。其中，我们研究了OsIDD 2，锌指和不确定结构域（IDD）家族TF的作用。其过表达植株表现为矮化、叶片脆弱和木质素含量降低，这些是SCW形成缺陷植株的典型表型，而其敲除植株表现为木质素含量略有增加。RNA-seq和定量逆转录聚合酶链反应分析证实，一些木质素生物合成基因在OsIDD 2过表达的植物中下调，并揭示了与纤维素合成和蔗糖代谢有关的其他基因的相同情况。利用水稻原生质体进行的瞬时表达实验表明，OsIDD 2负调控参与木质素生物合成的基因肉桂醇脱氢酶2和3的转录（CAD 2和3），和蔗糖代谢，蔗糖合成酶5（SUS 5），而AlphaScreen测定，其可以检测TF和它们的靶DNA序列之间的相互作用，直接证实了OsIDD 2与位于CAD 2和CAD 3启动子区的靶序列之间的相互作用。基于这些观察结果，我们得出结论，OsIDD 2是负性参与SCW的形成和其他生物学事件通过下调其靶基因。
Previously, we found 123 transcription factors (TFs) as candidate regulators of secondary cell wall (SCW) formation in rice by using phylogenetic and co-expression network analyses. Among them, we examined in this work the role of OsIDD2, a zinc finger and indeterminate domain (IDD) family TF. Its overexpressors showed dwarfism, fragile leaves, and decreased lignin content, which are typical phenotypes of plants defective in SCW formation, whereas its knockout plants showed slightly increased lignin content. The RNA-seq and quantitative reverse transcription polymerase chain reaction analyses confirmed that some lignin biosynthetic genes were downregulated in the OsIDD2-overexpressing plants, and revealed the same case for other genes involved in cellulose synthesis and sucrose metabolism. The transient expression assay using rice protoplasts revealed that OsIDD2 negatively regulates the transcription of genes involved in lignin biosynthesis, cinnamyl alcohol dehydrogenase 2 and 3 (CAD2 and 3), and sucrose metabolism, sucrose synthase 5 (SUS5), whereas an AlphaScreen assay, which can detect the interaction between TFs and their target DNA sequences, directly confirmed the interaction between OsIDD2 and the target sequences located in the promoter regions of CAD2 and CAD3. Based on these observations, we conclude that OsIDD2 is negatively involved in SCW formation and other biological events by downregulating its target genes.