CD45-positive cells of the retina and their responsiveness to in vivo and in vitro treatment with IFN-γ or anti-CD40
CD45-positive cells of the retina and their responsiveness to in vivo and in vitro treatment with IFN-γ or anti-CD40
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DOI：
10.1167/iovs.02-1014
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发表时间：
2003-07-01
影响因子：
4.4
通讯作者：
Yang, J
中科院分区：
文献类型：
--
作者：
Gregerson, DS;Yang, J
目的.在视网膜中产生抗原特异性免疫反应的能力证明了视网膜抗原的局部识别。然而，视网膜的抗原呈递细胞（APC）的性质是不确定的。本研究旨在寻找视网膜中具有APC潜能的CD 45（+）细胞的证据，并检测其对IFN-γ和抗CD 40的原位和体外反应，这两种刺激物已知上调与抗原呈递相关的活性。用IFN-γ或抗CD 40的全身或前房内（IC）接种预处理小鼠。收获视网膜，酶促解离，用抗CD 45阳性选择，并通过流式细胞术用已知识别APC的抗体进行分析。最常见的CD 45（+）视网膜细胞为CD 11b（+）、F4/80（+）、CD 8 α（+）、CD 80（+）和主要组织相容性复合物（MHC）IIlo类，这是中枢神经系统（CNS）小胶质细胞（MG）的特征表型。还有少量DEC-205（+）细胞和少量CD 11 c（+）细胞，这两种细胞都是树突状细胞（DC）的标志物。IC接种IFN-γ导致CD 45（+）细胞数量增加，CD 11b（+）细胞上的MHC II类分子适度上调。IC接种抗CD 40抗体也增加了CD 45+细胞总数和CD 11b（+）细胞数量，但对CD 11b（+）细胞上的CD 80和MHC II类表达的增加不明显。抗CD 40抗体处理后，CD 45（hi）11 c（+）细胞数量增加，CD 11b表达改变。视网膜MG容易被确定为最多的人群。发现了一小群具有血管周细胞（PVC）样性质的细胞。它们是CD 45（hi）11 c（+），有些具有升高的MHC II类，并且它们受到体内抗CD 40处理的影响。未发现常规DC，尽管存在明显的DEC-205（+）群体。总体而言，与对照组相比，IFN-γ和抗CD 40治疗在体内视网膜和培养的CD 45（+）细胞中的作用减弱。
PURPOSE. The ability to mount antigen-specific immune reactions in the retina demonstrates local recognition of retinal antigens. However, properties of antigen-presenting cells (APCs) of the retina are uncertain. The current study was undertaken to look for evidence of CD45(+) cells with APC potential in the retina and to examine their in situ and in vitro responses to IFN-gamma and anti-CD40, two stimuli known to up-regulate activities associated with antigen presentation.METHODS. Mice were pretreated with systemic or intracameral (IC) inoculations of IFN-gamma or anti-CD40. Retinas were harvested, enzymatically dissociated, positively selected with anti-CD45, and analyzed by flow cytometry with antibodies known to identify APCs.RESULTS. The most common CD45(+) retinal cells were CD11b(+), F4/80(+), CD8alpha(+), CD80(+), and major histocompatibility complex (MHC) class IIlo, a phenotype characteristic of central nervous system (CNS) microglia (MG). There was also a small population of DEC-205(+) cells and a smaller number of CD11c(+) cells, both markers of dendritic cells (DCs). IC inoculation of IFN-gamma led to an increase in the number of CD45(+) cells and a modest upregulation of MHC class II on CD11b(+) cells. IC inoculation of anti-CD40 also increased the total number of CD45+ cells and the number of CD11b(+) cells, but increases in CD80 and MHC class II expression on CD11b(+) cells were insignificant. After anti-CD40 treatment, CD45(hi)11c(+) cells increased in number and altered their expression of CD 11b.CONCLUSIONS. Retinal MG were readily identified as the most numerous population. A small population of cells with perivascular cell (PVC)-like properties was found. They were CD45(hi)11c(+), some had elevated MHC class II, and they were affected by anti-CD40 treatment in vivo. No conventional DCs were found, although there was a distinct DEC-205(+) population. Overall, the effects of IFN-gamma and anti-CD40 treatment were attenuated in the retina in vivo and also on CD45(+) cells in culture, compared with the control.