Development of Digestive System and Digestive Enzyme Activities of Larval and Juvenile Bluefin Tuna, Thunnus thynnus, Reared in the Laboratory
Development of Digestive System and Digestive Enzyme Activities of Larval and Juvenile Bluefin Tuna, Thunnus thynnus, Reared in the Laboratory
复制标题
实验室饲养蓝鳍金枪鱼幼鱼消化系统发育及消化酶活性
DOI：
10.11233/aquaculturesci1953.46.111
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
H. Kumai
中科院分区：
文献类型：
--
作者：
S. Miyashita;Keitaro Kato;Yoshifumi Sawada;O. Murata;Yutaka Ishitani;Kiyokazu Shimizu;Shinji Yamamoto;H. Kumai
研究了实验室人工饲养的蓝鳍金枪鱼幼鱼消化系统的发育及类胰蛋白酶、类胃蛋白酶和淀粉酶活性的变化。肝、胆、胰和肠直肠分界区在孵化后36 h内形成。开始投喂后，类胰蛋白酶和淀粉酶活性随着幼虫的生长而增加。在前屈阶段（孵化后10天内），肠的革命结束，咽齿和食管粘液细胞分化。在屈曲期（孵化后11 ~ 17天），出现功能性颌齿，盲囊、胃腺和幽门盲囊开始形成。胃蛋白酶样酶活性增加，胃和幽门盲囊的功能从后屈曲期发展到过渡期，直至幼鱼（出壳后17 ~ 25天）。肛前长占标准长的比例在出壳后11天内基本保持在40%左右，26天上升到65%，26 ~ 30天没有变化。这些结果表明，消化系统的发展，直到11日龄（前屈阶段）主要是定性的，从那时到26日龄（屈曲阶段后屈曲阶段）的定性和定量。消化系统的这种质和量的发展可能有助于幼鱼的快速生长。
Development of digestive system and changes in the activity of trypsin-like enzyme, pepsin-like enzyme and amylase were studied in larval and juvenile Bluefin tuna, Thunnus thynnus reared in the laboratory. Liver, gall bladder, pancreas, and the demarcating region between intestine and rectum formed within 36 h after hatching. After feeding commenced trypsin-like enzyme and amylase activities increased as the larvae grew. During the preflexion phase (within 10 days after hatching), revolution of the intestine concluded; and pharyngeal teeth and mucous cells of esophagus differentiated. During the flexion phase (11 to 17 days after hatching), functional jaw teeth were found and blind sac, gastric glands and pyloric caeca begin to form. Pepsin-like enzyme activity increased and functions of stomach and pyloric caeca developed from the postflexion phase to the transitional period to the juvenile (17 to 25 days after hatching). The rate of percentage of preanal length to standard length was constant (around 40%) until 11 days after hatching, then increased to 65% at day 26, and did not change from 26 days to 30 days. These results suggest that the developments of the digestive system until 11 days (preflexion phase) are mainly qualitative and those from then to 26 days (flexion phase to postflexion phase) are both qualitative and quantitative. This quantitative and qualitative development of the digestive system might contribute to the rapid growth in the juvenile stage.