Pulse Field Gel Electrophoresis.
Pulse Field Gel Electrophoresis.
复制标题
脉冲场凝胶电泳。
DOI：
10.1007/7651_2014_191
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
FowlerJr,VanceG
中科院分区：
文献类型：
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作者：
Sharma-Kuinkel,BatuK;Rude,ThomasH;FowlerJr,VanceG
脉冲场凝胶电泳 (PFGE) 是一种强大的基因分型技术，用于在用独特的限制性酶消化大 DNA 分子（整个基因组 DNA）并将其应用于周期性改变方向的电场下的凝胶基质后进行分离。 PFGE 是琼脂糖凝胶电泳的一种变体，可以分析比传统限制性酶分析大一个数量级的细菌 DNA 片段。它在单个凝胶中很好地展示了整个细菌染色体，具有高度可重复的限制性图谱，提供了清晰独特且分辨率良好的 DNA 片段。
Pulse Field Gel Electrophoresis (PFGE) is a powerful genotyping technique used for the separation of large DNA molecules (entire genomic DNA) after digesting it with unique restriction enzymes and applying to a gel matrix under the electric field that periodically changes direction. PFGE is a variation of agarose gel electrophoresis that permits analysis of bacterial DNA fragments over an order of magnitude larger than that with conventional restriction enzyme analysis. It provides a good representation of the entire bacterial chromosome in a single gel with a highly reproducible restriction profile, providing clearly distinct and well-resolved DNA fragments.