Cardiac troponin T isoform expression correlates with pathophysiological descriptors in patients who underwent corrective surgery for congenital heart disease.
Cardiac troponin T isoform expression correlates with pathophysiological descriptors in patients who underwent corrective surgery for congenital heart disease.
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心脏肌钙蛋白 T 同工型表达与接受先天性心脏病矫正手术的患者的病理生理学描述相关。
DOI：
10.1161/01.cir.94.3.472
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发表时间：
1996
期刊：
影响因子：
37.8
通讯作者：
Anderson,PA
中科院分区：
文献类型：
--
作者：
Saba,Z;Nassar,R;Ungerleider,RM;Oakeley,AE;Anderson,PA
背景本研究检测了1993年12月1日至1995年1月31日在杜克大学医学中心（达勒姆，NC）接受先天性心脏病矫正手术的患者的心肌肌钙蛋白T（cTnT）亚型表达。人心脏表达四种cTnT亚型（cTnT 1至cTnT 4），其序列差异是由两个外显子的组合选择性剪接引起的。我们先前已经证明，cTnT 4在移植患者严重衰竭的心脏中表达水平较高。在这项研究中，我们验证了先天性心脏病对心肌功能有更多负面影响的假设，即cTnT 4表达增加。我们使用的存在或不存在的心脏衰竭或充血循环的药物治疗手术前和矫正手术后的正性肌力支持的持续时间作为指标的心脏的病理生理状态的前surgery.Methods和ResultsRight心耳组织收集了34例患者，6天至35岁（平均年龄，3.4个月）。cTnT特异性单克隆抗体MAb 13 -11通过蛋白质印迹法测定cTnT 1-cTnT 4亚型的量（以占总cTnT的百分比计）。我们发现cTnT 4表达与正性肌力支持的持续时间呈正相关，术前接受药物治疗的患者cTnT 4表达高于未接受药物治疗的患者。此外，我们发现，cTnT 4的百分比是显着较高的心脏先天性缺陷，造成充血性心力衰竭比心脏法洛四联症。ConclusionsThese研究结果表明，在先天性心脏缺陷的患者，cTnT 4的表达是由心力衰竭调制，并增加了心脏的血流动力学压力。
BackgroundThis study examined cardiac troponin T (cTnT) isoform expression in patients who had undergone surgery at Duke University Medical Center (Durham, NC) between December 1, 1993, and January 31, 1995, to correct congenital heart defects. The human heart expresses four cTnT isoforms (cTnT1through cTnT4) whose sequence differences result from combinatorial alternative splicing of two exons. We have previously shown that cTnT4is expressed at higher levels in severely failing hearts from transplant patients. In this study, we tested the hypothesis that congenital heart defects that have a more negative effect on myocardial function increase cTnT4expression. We used the presence or absence of drug treatment for heart failure or congested circulation before surgery and the duration of inotropic support after corrective surgery as indicators of the pathophysiological state of the heart just before surgery.Methods and ResultsRight atrial appendage tissue was collected from 34 patients, 6 days to 35 years old (median age, 3.4 months). The amounts of the cTnT1through cTnT4isoforms, measured as a percentage of total cTnT, were determined from Western blots probed with MAb13-11, a cTnT-specific monoclonal antibody. We found that cTnT4expression correlated positively with the duration of inotropic support and was higher in patients who received drug treatment before surgery than in those who did not. Furthermore, we found that the percent of cTnT4was significantly higher in hearts with congenital defects that caused congestive failure than in hearts with tetralogy of Fallot.ConclusionsThese findings suggest that in patients with congenital cardiac defects, cTnT4expression is modulated by heart failure and is increased in hearts that are more hemodynamically stressed.
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DOI：
--
发表时间：
1988-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Jin;J. Lin
通讯作者：
J. Jin;J. Lin
兔心肌力-pCa 关系和肌钙蛋白 T 亚型。
影响因子：
20.1
作者：
Nassar,R;Malouf,NN;Kelly,MB;Oakeley,AE;Anderson,PA
通讯作者：
Anderson,PA
影响因子：
20.1
作者：
ANDERSON, PAW;MALOUF, NN;ALLEN, PD
通讯作者：
ALLEN, PD
牛心肌肌钙蛋白 T 亚型的分离和功能比较。
影响因子：
4.8
作者：
Larry S. TobacmanS;R. Lee
通讯作者：
R. Lee
先天性心脏病手术术中超声心动图学习曲线
DOI：
10.1016/0003-4975(92)91013-y
发表时间：
1992
期刊：
The Annals of Thoracic Surgery
影响因子：
--
作者：
R. Ungerleider;W. Greeley;R. Kanter;J. Kisslo
通讯作者：
J. Kisslo