iTRAQ-based quantitative proteomic analysis of the global response to 17β-estradiol in estrogen-degradation strain Pseudomonas putida SJTE-1.
iTRAQ-based quantitative proteomic analysis of the global response to 17β-estradiol in estrogen-degradation strain Pseudomonas putida SJTE-1.
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基于 iTRAQ 的雌激素降解菌株恶臭假单胞菌 SJTE-1 对 17 β-雌二醇整体反应的定量蛋白质组学分析
DOI：
10.1038/srep41682
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发表时间：
2017-02-03
影响因子：
4.6
通讯作者：
Liang R
中科院分区：
文献类型：
--
作者：
Xu J;Zhang L;Hou J;Wang X;Liu H;Zheng D;Liang R
微生物降解对17β-雌二醇（E2）等甾体激素的去除是有效的，但其降解机理和代谢网络尚不十分清楚。采用iTRAQ（isobaric tags for relative and absolute quantization）结合LC-MS/MS（liquid chromatography-tandem mass spectrometry）的方法，分析比较了雌激素降解菌恶臭假单胞菌SJTE-1对17β-雌二醇和葡萄糖的整体响应。共鉴定出78个蛋白表达发生显著变化，其中上调蛋白45个，下调蛋白33个。这些蛋白质主要参与应激反应、能量代谢、运输、趋化性和细胞运动以及碳代谢等过程，被认为可能对17β-雌二醇产生应答并在其代谢中发挥作用。电子传递、能量产生和转运系统中的上调蛋白被认为是17β-雌二醇有效摄取、转运和转化的关键。碳代谢蛋白的过表达表明细胞可能激活相关通路成员以利用17β-雌二醇。与此同时，葡萄糖捕获和代谢的蛋白质大多下调。这些研究结果为揭示恶臭假单胞菌降解17β-雌二醇的机理及促进其在生物修复中的应用提供了重要线索。
Microorganism degradation is efficient to remove the steroid hormones like 17β-estradiol (E2); but their degradation mechanism and metabolic network to these chemicals are still not very clear. Here the global responses of the estrogen-degradation strain Pseudomonas putida SJTE-1 to 17β-estradiol and glucose were analyzed and compared using the iTRAQ (isobaric tags for relative and absolute quantization) strategy combined with LC-MS/MS (liquid chromatography-tandem mass spectrometry). 78 proteins were identified with significant changes in expression; 45 proteins and 33 proteins were up-regulated and down-regulated, respectively. These proteins were mainly involved in the processes of stress response, energy metabolism, transportation, chemotaxis and cell motility, and carbon metabolism, considered probably responding to 17β-estradiol and playing a role in its metabolism. The up-regulated proteins in electron transfer, energy generation and transport systems were thought crucial for efficient uptake, translocation and transformation of 17β-estradiol. The over-expression of carbon metabolism proteins indicated cells may activate related pathway members to utilize 17β-estradiol. Meanwhile, proteins functioning in glucose capture and metabolism were mostly down-regulated. These findings provide important clues to reveal the 17β-estradiol degradation mechanism in P. putida and promote its bioremediation applications.