Spatial distribution of arsenic and temporal variation of its concentration in rice.
Spatial distribution of arsenic and temporal variation of its concentration in rice.
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DOI：
10.1111/j.1469-8137.2010.03456.x
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发表时间：
2011
期刊：
影响因子：
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通讯作者：
Maozhong Zheng;Chao Cai;Ying Hu;Guoxin Sun;Paul N. Williams;H. Cui;Gang Li;F. Zhao;Yong-guan Zhu-Yo
中科院分区：
文献类型：
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作者：
Maozhong Zheng;Chao Cai;Ying Hu;Guoxin Sun;Paul N. Williams;H. Cui;Gang Li;F. Zhao;Yong-guan Zhu-Yo
为了研究砷在水稻植株体内的迁移、分布及其向籽粒中的卸载，研究了不同生育期水稻植株体内砷的空间分布和浓度的时间变化。据我们所知，这是第一次进行这样的研究。·采用电感耦合等离子体质谱（ICP-MS）和高效液相色谱（HPLC）-ICP-MS分析总砷浓度和形态。此外，同步辐射X射线荧光（SXRF）原位研究了砷在叶片，节间，节和籽粒中的分布。营养组织中总砷含量在开花后2周内呈上升趋势。随着颖果的发育，颖果中二甲基胂酸（DMA）的浓度逐渐降低，而无机砷的浓度保持稳定。相邻叶片间和相邻节间间的砷含量比值为c。0.6. SXRF分析表明，砷在颖果发育早期积累在颖果中心，然后积累在胚珠维管中。这些结果表明，无机As和DMA的卸载受不同的控制，后者在开花前主要在颖果中积累，而无机As主要在灌浆期向颖果中转运。此外，节点似乎作为一个检查点在水稻芽中的分配。
• In order to gain insights into the transport and distribution of arsenic (As) in intact rice (Oryza sativa) plants and its unloading into the rice grain, we investigated the spatial distribution of As and the temporal variation of As concentration in whole rice plants at different growth stages. To the best of our knowledge, this is the first time that such a study has been performed. • Inductively coupled plasma mass spectroscopy (ICP-MS) and high-performance liquid chromatography (HPLC)-ICP-MS were used to analyze total As concentration and speciation. Moreover, synchrotron-based X-ray fluorescence (SXRF) was used to investigate in situ As distribution in the leaf, internode, node and grain. • Total As concentrations of vegetative tissues increased during the 2 wk after flowering. The concentration of dimethylarsinic acid (DMA) in the caryopsis decreased progressively with its development, whereas inorganic As concentration remained stable. The ratios of As content between neighboring leaves or between neighboring internodes were c. 0.6. SXRF revealed As accumulation in the center of the caryopsis during its early development and then in the ovular vascular trace. • These results indicate that there are different controls on the unloading of inorganic As and DMA; the latter accumulated mainly in the caryopsis before flowering, whereas inorganic As was mainly transported into the caryopsis during grain filling. Moreover, nodes appeared to serve as a check-point in As distribution in rice shoots.