Peptide ormosils as cellular substrates
Peptide ormosils as cellular substrates
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DOI：
10.1039/b705393b
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发表时间：
2007-01-01
影响因子：
--
通讯作者：
Rickus, Jenna L.
中科院分区：
文献类型：
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作者：
Jedlicka, Sabrina S.;Little, Kenneth M.;Rickus, Jenna L.
肽功能化薄膜显示出整合到可植入装置和基于细胞的技术中的显著潜力。利用溶胶-凝胶反应化学，从四种不同的肽硅烷前体制备薄膜，开发了一种新型的神经活性肽改性二氧化硅。使用标准固态FMOC肽合成条件和3 '-（氨基丙基）三甲氧基硅烷（APTMS）的共价连接，使用羰基二咪唑（CDI）作为连接分子，产生含有来自层粘连蛋白（YIGSR和KDI）、纤连蛋白（RGD）和来自层粘连蛋白和腱生蛋白（NID）的EGF重复的结合序列的肽硅烷。用MALDI-MS确认前体形成。使用与水解的四甲氧基硅烷组合的肽前体通过浸涂制备薄膜。原子力显微镜表明，表面形貌不受低浓度的肽前体（0.0025摩尔%）的影响，但较高浓度的肽前体（0.01摩尔%）导致高度为50 - 75 nm的特征。观察到的高度特征在尺寸上与先前确定的支持神经元细胞系的地形形态一致。这些表面具有生物活性，并调节胚胎癌干细胞系P19的表型。肽硅烷的组合导致在视黄酸处理后改变的细胞类型。与四甲氧基硅烷（TMOS）对照相比，在RGD/YIGSR和RGD/YIGSR/NID表面上观察到更多的神经元。与胶原包被的组织培养板相比，观察到更多的支持细胞。此外，与对照组相比，肽ormosil上的神经突显着更长。这项工作展示了一种新的方法，用于生产生物活性肽ormosil使用肽修饰的前体。
Peptide-functionalized thin films exhibit significant potential for integration into implantable devices and cell-based technologies. A new type of neuroactive peptide-modified silica was developed using sol-gel reaction chemistry to produce thin films from four different peptide silane precursors. Peptide silanes containing binding sequences from laminin ( YIGSR and KDI), fibronectin ( RGD), and EGF repeats from laminin and tenascin ( NID) were produced using standard solid-state FMOC peptide synthesis conditions and the covalent attachment of 3'-( aminopropyl) trimethoxysilane ( APTMS), using carbonyldiimadazole ( CDI) as a linking molecule. Precursor formation was confirmed with MALDI- MS. Thin films were produced by dip-coating using the peptide precursors combined with hydrolyzed tetramethoxysilane. Atomic force microscopy indicated that the surface topography was not affected by low concentrations of peptide precursor ( 0.0025 mol%), but higher concentrations of peptide precursor ( 0.01 mol%) resulted in features that were 50 - 75 nm in height. The height features observed were consistent in size with previously determined topographical morphology supportive of neuronal cell lines. The surfaces were biologically active and modulated the phenotype of the embryonic carcinoma stem cell line, P19. Combinations of the peptide silanes resulted in altered cell types after retinoic acid treatment. More neurons were observed on RGD/YIGSR and RGD/YIGSR/NID surfaces compared to tetramethoxysilane ( TMOS) controls. More supporting cells were observed compared to collagen coated tissue culture plates. In addition, neurites were significantly longer on the peptide ormosils compared to controls. This work demonstrates a novel method for producing biologically active peptide ormosils using peptide-modified precursors.