Melittin selectively activates capsaicin-sensitive primary afferent fibers
Melittin selectively activates capsaicin-sensitive primary afferent fibers
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DOI：
10.1097/01.wnr.0000135919.37807.a7
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发表时间：
2004-08-06
期刊：
影响因子：
1.7
通讯作者：
Kim, JH
中科院分区：
文献类型：
--
作者：
Shin, HK;Kim, JH
全蜂毒（WBV）致痛模型已被报道是非常有用的疼痛研究。然而，WBV引起疼痛的主要成分并不明显。足底注射WBV和蜂毒肽显著降低机械阈值，并增加退缩和爪厚度。在行为实验中，辣椒素预处理几乎完全防止WBV和蜂毒肽诱导的机械阈值和退缩的减少。足底注射蜂毒肽仅能增加外周感受野C纤维传入的背角神经元放电频率，而坐骨神经局部应用辣椒素可完全阻断该作用。这些结果表明，蜂毒肽和WBV诱导辣椒素敏感的传入纤维的选择性激活伤害性反应。
Whole bee venom (WBV)-induced pain model has been reported to be very useful for the study of pain. However, the major constituent responsible for the production of pain by WBV is not apparent. Intraplantar injection of WBV and melittin dramatically reduced mechanical threshold, and increased flinchings and paw thickness. In behavioral experiments, capsaicin pretreatment almost completely prevented WBV- and melittin-induced reduction of mechanical threshold and flinchings. Intraplantar injection of melittin increased discharge rate of dorsal horn neurons only with C fiber input from peripheral receptive field, which was completely blocked by topical application of capsaicin to sciatic nerve. These results suggest that both melittin and WBV induce nociceptive responses by selective activation of capsaicin-sensitive afferent fibers.