Acute effect of electroacupuncture at the Zusanli acupoints on decreasing insulin resistance as shown by lowering plasma free fatty acid levels in steroid-background male rats
Acute effect of electroacupuncture at the Zusanli acupoints on decreasing insulin resistance as shown by lowering plasma free fatty acid levels in steroid-background male rats
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DOI：
10.1186/1472-6882-9-26
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发表时间：
2009-08-01
影响因子：
--
通讯作者：
Chang, Shih-Liang
中科院分区：
文献类型：
--
作者：
Lin, Rong-Tsung;Tzeng, Chung-Yuh;Chang, Shih-Liang
背景资料：电针可增强大鼠的胰岛素敏感性，但胰岛素抵抗状态下的电针现象尚不清楚。本研究报告了使用大剂量的泼尼松龙，以评估EA在胰岛素抵抗状态下的影响。方法：血浆游离脂肪酸（FFAs）水平估计类固醇背景大鼠（SBRs），并与那些在健康大鼠用生理盐水治疗。此外，测定血浆葡萄糖和内源性胰岛素水平以计算稳态模型评估（HOMA）指数。采用静脉葡萄糖耐量试验（IVGTT）比较两组的糖耐量。随机分为电针治疗组和非电针治疗组，采用15 Hz电针双侧足三里穴，观察电针对胰岛素抵抗的影响。结果：实验组血浆游离脂肪酸（FFA）水平明显升高，HOMA指数明显升高，糖耐量受损。电针通过降低血浆游离脂肪酸水平改善糖耐量和胰岛素敏感性。结论：大剂量泼尼松龙可成功诱导雄性大鼠胰岛素抵抗。15 Hz电针双侧足三里穴可改善胰岛素抵抗，降低血浆游离脂肪酸水平。
Background: Insulin sensitivity has been enhanced by electroacupuncture ( EA) in rats, but the EA phenomenon in an insulin resistant state is still unclear. This study reports the use of a large dose of prednisolone to evaluate the effects of EA in a state of insulin resistance.Methods: The plasma levels of free fatty acids (FFAs) were estimated in steroid-background rats (SBRs) and compared with those in healthy rats treated with normal saline. In addition, plasma glucose and endogenous insulin levels were assayed to calculate the homeostasis model assessment (HOMA) index. Intravenous glucose tolerance test (IVGTT) was carried out to compare glucose tolerance. The SBRs were randomly divided into EA-treatment and non-EA treatment groups and 15-Hz EA was applied to the bilateral Zusanli acupoints to investigate its effects on insulin resistance. In addition to an insulin challenge test (ICT) and IVGTT, the plasma levels of FFAs were measured and western blot was performed to help determine the effects of EA on the insulin resistant state.Results: The plasma levels of FFAs increased markedly in SBRs, the HOMA index was markedly higher, and glucose tolerance was impaired. EA improved glucose tolerance and insulin sensitivity by decreasing the plasma levels of FFAs. Further, the insulin signaling proteins (IRSI) and glucose transporter isoform protein ( GLUT4) in skeletal muscle inhibited by prednisolone recovered after EA.Conclusion: Insulin resistance was successfully induced by a large dose of prednisolone in male rats. This insulin resistance can be improved by 15 Hz EA at the bilateral Zusanli acupoints, as shown by decreased plasma levels of FFAs.