Effects of season and artificial photoperiod on semen and seminal plasma characteristics in bucks of two goat breeds maintained in a semen collection center.
Effects of season and artificial photoperiod on semen and seminal plasma characteristics in bucks of two goat breeds maintained in a semen collection center.
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DOI：
10.14202/vetworld.2017.521-525
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发表时间：
2017-05
期刊：
影响因子：
1.6
通讯作者：
Abecia JA
中科院分区：
文献类型：
--
作者：
Arrebola F;Abecia JA
本研究量化了过去一年中季节和光周期处理对两个西班牙山羊品种(Murciano-Granadina和Payoya)12头山羊精液和精浆(SP)特性的影响。总共6只雄鹿(每种3只)暴露在自然日长下，其余6只雄鹿(每种3只)暴露在2个月长日照(16h光照)和2个月短日照(8h光照)交替条件下。每周测定SP中谷草转氨酶/天冬氨酸氨基转移酶(GOT/AST)、乳酸脱氢酶(LDH)、钾、睾酮和蛋白质的浓度。在精液采集前记录反应时间和阴囊周长，并测定血浆睾酮浓度。精子体积、乳酸脱氢酶、SP中的钾浓度和反应时间在品种、季节和光周期处理之间没有显著差异。精子密度在春季和夏季高于秋季和冬季(p<；0.001)。低渗肿胀试验阳性精子的平均百分率在夏季和人工光周期下最高(p<；0.01)。谷草转氨酶/天冬氨酸氨基转移酶浓度因品种和季节的不同而不同(p<；0.01)。品种、季节和光周期对SP的蛋白质和睾酮水平有显著影响(p<；0.001)。血浆睾酮浓度在夏季最高(p<；0.001)，品种间差异显著(p<；0.01)。阴囊周长在品种和光周期之间存在显著差异(p<；0.001)。认识到这些季节性和特定品种的雄鹿性能差异，应该有助于改善人工授精项目中使用的个体精液样本的管理。
This study quantified the effects of season and photoperiodic treatment on semen and seminal plasma (SP) characteristics in 12 bucks of two Spanish goat breeds (Murciano-Granadina, and Payoya) for the past 1 year. A total of 6 bucks (three of each breed) were exposed to the natural day length and the other six males (three of each breed) were exposed to alternating conditions of 2 months of long days (16 h light) and 2 months of short days (8 h light). Weekly concentrations of glutamic oxaloacetic transaminase/aspartate aminotransferase (GOT/AST), lactate dehydrogenase (LDH), potassium, testosterone, and protein in SP were measured. Reaction time and scrotal circumference were recorded, and plasma testosterone concentrations were measured before semen collection. Sperm volume, LDH, and potassium concentration in SP, and reaction time did not differ significantly between breeds, seasons, and photoperiodic treatment. Sperm concentrations were higher (p<0.001) in spring and summer than they were in autumn and winter. Mean percentage of positive hypo-osmotic swelling test sperm was the highest in summer and under the artificial photoperiod (p<0.01). GOT/AST concentrations differed (p<0.01) between breeds and seasons. Breed, season, and photoperiod had significant (p<0.001) effects on protein and testosterone levels in SP. Plasma testosterone concentrations were highest in summer (p<0.001), and differed significantly (p<0.01) between breeds. Scrotal perimeter differed significantly (p<0.001) between breeds and photoperiod. Recognition of those seasonal and breed-specific differences in the performance of bucks should help to improve the management of individual semen samples for use in artificial insemination programs.