HIV-1 and HIV-2 infections in a high-risk population in Bombay, India: evidence for the spread of HIV-2 and presence of a divergent HIV-1 subtype.
HIV-1 and HIV-2 infections in a high-risk population in Bombay, India: evidence for the spread of HIV-2 and presence of a divergent HIV-1 subtype.
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印度孟买高危人群中的 HIV-1 和 HIV-2 感染：HIV-2 传播和不同 HIV-1 亚型存在的证据。
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
H. Rübsamen
中科院分区：
文献类型：
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作者：
A. Pfützner;U. Dietrich;U. von Eichel;H. von Briesen;H. Brede;J. Maniar;H. Rübsamen
对高危人群(孟买一家性传播疾病诊所和GT医院的患者)进行了艾滋病毒-1和艾滋病毒-2抗体检测。在405份血清样本中，有226份之前在印度使用不同的当地可获得的酶联免疫吸附试验(ELISA)被归类为艾滋病毒阳性。179份样本的血清学尚不清楚。所有405份样本都在Georg-Speyer-Haus(GSH)用巴斯德HIV-1/2-Combi-ELISA进行了检测。阳性标本分别用杜邦和巴斯德的HIV-1和HIV-2蛋白印迹试剂盒进一步分析。在这一人群中发现了非常高的艾滋病毒血清阳性率。在179份未筛查的样本中，69份(38.5%)在ELISA和Western blotting中对HIV-1或HIV-2呈阳性。在预先筛查的样本中，只有174人(77%)被确认为艾滋病毒阳性。总体而言，405份血清中有243份艾滋病毒呈阳性。其中，184例(76%)对HIV-1反应，10例(4%)对HIV-2反应，49例(20%)对HIV-1和HIV-2双重反应。印度医学研究委员会此前的数据已经表明，印度艾滋病毒-1的流行率可能很高。我们的结果证实了这一观点。然而，在印度发现艾滋病毒-2感染的大量传播是完全出乎意料的，但证实了我们之前的研究，该研究已经证明了艾滋病毒-2在印度的存在。因此，亚洲不再被认为没有艾滋病毒-2，艾滋病毒-2检测似乎是强制性的，至少在印度是这样。
A high-risk population (patients of a sexually transmitted disease clinic and the GT hospital in Bombay) was tested for antibodies against HIV-1 and HIV-2. Among 405 serum samples, 226 had previously been classified HIV-positive in India using different locally available enzyme-linked immunosorbent assay (ELISA) tests. The serology of 179 samples was unknown. All 405 samples were tested at the Georg-Speyer-Haus (GSH) with the Pasteur HIV-1/2-Combi-ELISA. Positive samples were further analyzed with HIV-1 and HIV-2 Western blot kits from Dupont and Pasteur, respectively. A very high seroprevalence of HIV was found in this population. Among the 179 unscreened samples, 69 (38.5%) were positive in the ELISAs as well as the Western blots for HIV-1 or HIV-2. Among the prescreened samples, only 174 (77%) were confirmed HIV-positive. Altogether, 243 of 405 sera were HIV-positive. Of these, 184 (76%) were reactive with HIV-1, 10 (4%) were reactive with HIV-2, and 49 (20%) had dual reactivity to HIV-1 and HIV-2. Previous data from the Indian Council of Medical Research had already suggested a possible high prevalence of HIV-1 in India. Our results confirm this view. The finding of a substantial spread of HIV-2 infection was, however, totally unexpected in India, but confirms our previous study which had already demonstrated the existence of HIV-2 in this country. Asia can thus no longer be considered free of HIV-2, and testing for HIV-2 appears mandatory, at least in India.(ABSTRACT TRUNCATED AT 250 WORDS)