Generation of concanavalin A-induced suppressor cells in the cat.
Generation of concanavalin A-induced suppressor cells in the cat.
复制标题
猫体内伴刀豆球蛋白 A 诱导的抑制细胞的生成。
DOI：
--
复制
发表时间：
1982
期刊：
影响因子：
--
通讯作者：
G. Cockerell
中科院分区：
文献类型：
--
作者：
M. Langweiler;G. Cockerell
当猫外周血单个核细胞与未经处理的自体细胞共培养时，用有丝分裂剂量的刀豆蛋白A预活化的猫外周血单核细胞可抑制增殖。抑制程度与预激活时间和预激活与未处理细胞的比例成正比。抑制细胞的产生，而不是活性，依赖于DNA和蛋白质的合成。商陆有丝分裂原和植物血凝素也被证明能够诱导抑制细胞活性。在同种异体共培养中也观察到抑制。刀豆蛋白A预激活的细胞培养上清不能抑制未经处理的细胞的增殖。
Preactivation of feline peripheral blood mononuclear cells with mitogenic doses of concanavalin A resulted in suppression of proliferation when these cells were cocultured with untreated autologous cells. The degree of suppression was directly proportional to the preactivation time and ratio of preactivated to untreated cells. The generation, but not the activity, of the suppressor cells was dependent on DNA and protein synthesis. Pokeweed mitogen and phytohemagglutinin were also shown to be capable of inducing suppressor cell activity. Suppression was also observed in allogeneic cocultures. Supernatants from concanavalin A-preactivated cell cultures did not suppress proliferation of untreated cells.