AN IMPROVED METHOD FOR THE MEASUREMENT OF MALONDIALDEHYDE IN BIOLOGICAL SAMPLES
AN IMPROVED METHOD FOR THE MEASUREMENT OF MALONDIALDEHYDE IN BIOLOGICAL SAMPLES
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DOI：
10.1007/bf02537094
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发表时间：
1994-01-01
期刊：
影响因子：
1.9
通讯作者：
CHOW, CK
中科院分区：
文献类型：
--
作者：
LI, XY;CHOW, CK
建立了一种改进的以硫代巴比妥酸（TBA）络合物形式测定丙二醛（MDA）的方法。样品首先与1%碘化钾和0.1%丁基化羟基甲苯在50 ℃下孵育20 min，然后与0.4% TBA在60 ℃下孵育60 min。形成的MDA-TBA复合物用异丁醇提取，并通过高效液相色谱法与荧光检测器进行测量。改进的方法允许更具体地测定生物样品中存在的MDA。
An improved method was developed for measuring malondialdehyde (MDA) as its thiobarbituric acid (TBA) complex. Samples were initially incubated with 1% potassium iodide and 0.1% butylated hydroxytoluene at 50 degrees C for 20 min, and then with 0.4% TBA at 60 degrees C for 60 min. The MDA-TBA complex formed was extracted with isobutyl alcohol and measured by high performance liquid chromatography with fluorescence detection. The improved method allows for a more specific determination of MDA present in biological samples.