Expression of Clarkia S-linalool synthase in transgenic petunia plants results in the accumulation of S-linalyl-β-D-glucopyranoside
Expression of Clarkia S-linalool synthase in transgenic petunia plants results in the accumulation of S-linalyl-β-D-glucopyranoside
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DOI：
10.1046/j.1365-313x.2001.01097.x
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发表时间：
2001-08-01
期刊：
影响因子：
7.2
通讯作者：
Verhoeven, HA
中科院分区：
文献类型：
--
作者：
Lücker, J;Bouwmeester, HJ;Verhoeven, HA
将矮牵牛S芳樟醇合成酶基因(LIS)导入矮牵牛W115。LIS在所分析的所有组织中都有表达，芳樟醇在叶、花冠、花冠、茎和子房中检测到，而在蜜腺、根、花粉和花柱中没有检测到。然而，该植物在各种组织中产生的S-芳樟醇并不是以游离芳樟醇的形式存在，而是在内源葡萄糖转移酶的作用下有效地转化为非挥发性的S-芳樟醇-β-D-吡喃葡萄糖苷。结果表明，单萜的产生可以通过基因修饰来改变，所产生的化合物可以通过内源酶活性来转化。
Petunia hybrida W115 was transformed with a Clarkia breweri S-linalool synthase cDNA (lis). Lis was expressed in all tissues analysed, and linalool was detected in leaves, sepals, corolla, stem and ovary, but not in nectaries, roots, pollen and style. However, the S-linalool produced by the plant in the various tissues is not present as free linalool, but was efficiently converted to non-volatile S-linalyl-beta -D-glucopyranoside by the action of endogenous glucosyltransferase. The results presented demonstrate that monoterpene production can be altered by genetic modification, and that the compounds produced can be converted by endogenous enzymatic activity.